Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

Xu Ping Wang, Peng Fei Zhou, Zhi Gang Li, Bo Yang, Frank Hollmann, Yong Hua Wang*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)
28 Downloads (Pure)

Abstract

A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.

Original languageEnglish
Article number44599
Number of pages5
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 20 Mar 2017

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