Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

Xu Ping Wang; Peng Fei Zhou; Zhi Gang Li; Bo Yang; Frank Hollmann; Yong Hua Wang

doi:10.1038/srep44599

Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

Xu Ping Wang, Peng Fei Zhou, Zhi Gang Li, Bo Yang, Frank Hollmann, Yong Hua Wang^*

^*Corresponding author for this work

BT/Biocatalysis

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Abstract

A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.

Original language	English
Article number	44599
Number of pages	5
Journal	Scientific Reports
Volume	7
DOIs	https://doi.org/10.1038/srep44599
Publication status	Published - 20 Mar 2017

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abstract = "A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.",

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AU - Wang, Xu Ping

AU - Zhou, Peng Fei

AU - Li, Zhi Gang

AU - Yang, Bo

AU - Hollmann, Frank

AU - Wang, Yong Hua

PY - 2017/3/20

Y1 - 2017/3/20

N2 - A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.

AB - A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.

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Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

Abstract

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