TY - JOUR
T1 - Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium
AU - Muñiz-Márquez, Diana B.
AU - Contreras, Juan C.
AU - Rodríguez, Raúl
AU - Mussatto Dragone, Solange
AU - Teixeira, José A.
AU - Aguilar, Cristóbal N.
PY - 2016
Y1 - 2016
N2 - The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm3. Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.
AB - The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm3. Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.
KW - Aguamiel
KW - Fructooligosaccharides
KW - Fructosyltransferase
KW - Solid State Fermentation
UR - http://www.scopus.com/inward/record.url?scp=84962256750&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.03.022
DO - 10.1016/j.biortech.2016.03.022
M3 - Article
AN - SCOPUS:84962256750
SN - 0960-8524
VL - 213
SP - 276
EP - 282
JO - Bioresource Technology
JF - Bioresource Technology
ER -