Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

Diana B. Muñiz-Márquez, Juan C. Contreras, Raúl Rodríguez, Solange Mussatto Dragone, José A. Teixeira, Cristóbal N. Aguilar

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm3. Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.

Original languageEnglish
Pages (from-to)276-282
Number of pages7
JournalBioresource Technology
Volume213
DOIs
Publication statusPublished - 2016

Keywords

  • Aguamiel
  • Fructooligosaccharides
  • Fructosyltransferase
  • Solid State Fermentation

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