TY - JOUR
T1 - Enrichment and characterization of a psychrophilic ‘Candidatus Accumulibacter phosphatis’ culture
AU - Tian, W. D.
AU - Ma, C.
AU - Lin, Y. M.
AU - Welles, L.
AU - Lopez-Vazquez, C.
AU - van Loosdrecht, M. C.M.
PY - 2017
Y1 - 2017
N2 - Enhanced Biological phosphorous removal (EBPR) processes, often operated at low temperatures, are utilised world-wide, but currently little is known regarding enrichment cultures and the characteristics of active organisms (“Candidatus Accumulibacter phosphatis” (Accumulibacter)) under psychrophilic conditions. This study assesses the long-term performance, metabolic activity, microbial community characteristics and sludge morphology in an EBPR community enriched from activated sludge at 10 °C. Long solid retention times (SRT) and low temperatures resulted in the dominance of Accumulibacter type II over type I. Despite changes in the microbial community, P-removal efficiencies did not show obvious differences and although no specific measures were implemented, the enriched Accumulibacter-PAO culture formed stable dense granules. A high level of Alginate-like exopolysaccharides (ALE) were observed, with a large number of Guluronic acid-Guluronic acid (GG) blocks derived from the biomass at 10 °C. This characteristic favors sludge granulation, increasing the mechanical strength of granules formed, which encourages solid-liquid separation and consequently, contributes to the stable operation of EBPR systems.
AB - Enhanced Biological phosphorous removal (EBPR) processes, often operated at low temperatures, are utilised world-wide, but currently little is known regarding enrichment cultures and the characteristics of active organisms (“Candidatus Accumulibacter phosphatis” (Accumulibacter)) under psychrophilic conditions. This study assesses the long-term performance, metabolic activity, microbial community characteristics and sludge morphology in an EBPR community enriched from activated sludge at 10 °C. Long solid retention times (SRT) and low temperatures resulted in the dominance of Accumulibacter type II over type I. Despite changes in the microbial community, P-removal efficiencies did not show obvious differences and although no specific measures were implemented, the enriched Accumulibacter-PAO culture formed stable dense granules. A high level of Alginate-like exopolysaccharides (ALE) were observed, with a large number of Guluronic acid-Guluronic acid (GG) blocks derived from the biomass at 10 °C. This characteristic favors sludge granulation, increasing the mechanical strength of granules formed, which encourages solid-liquid separation and consequently, contributes to the stable operation of EBPR systems.
KW - Candidatus Accumulibacter phosphatis
KW - EBPR
KW - Low temperature
KW - Psychrophilic conditions
UR - http://www.scopus.com/inward/record.url?scp=85017136904&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2017.03.030
DO - 10.1016/j.ibiod.2017.03.030
M3 - Article
AN - SCOPUS:85017136904
SN - 0964-8305
VL - 124
SP - 267
EP - 275
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
ER -