Enzyme engineering for biocatalysis

Caroline Paul, Ulf Hanefeld, Frank Hollmann*, Ge Qu, Bo Yuan, Zhoutong Sun

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)
154 Downloads (Pure)

Abstract

Contemporary Biocatalysis heavily relies on enzyme engineering as natural enzymes frequently lack the requisite attributes for effective organic synthesis. The inherent limitations in stability, catalytic activity, and selectivity of wild-type enzymes often hinder their suitability for chemical synthesis. Over the past 25 years, there has been an unprecedented advancement in protein engineering tools, empowering enzymologists to customise enzymes to precisely meet the demands of organic synthesis. In this discussion, we delineate some of the most crucial techniques in enzyme engineering and their significance in facilitating chemical synthesis.

Original languageEnglish
Article number113874
Number of pages13
JournalMolecular Catalysis
Volume555
DOIs
Publication statusPublished - 2024

Keywords

  • Biocatalysis
  • Directed evolution
  • Iterative saturation mutagenesis
  • Semi-rational design

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