Estimation of microtubule-generated forces using a DNA origami nanospring

Ali Nick Maleki, Pim J. Huis In 't Veld, Anna Akhmanova, Marileen Dogterom, Vladimir A. Volkov

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)
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Microtubules are dynamic cytoskeletal filaments that can generate forces when polymerizing and depolymerizing. Proteins that follow growing or shortening microtubule ends and couple forces to cargo movement are important for a wide range of cellular processes. Quantifying these forces and the composition of protein complexes at dynamic microtubule ends is challenging and requires sophisticated instrumentation. Here, we present an experimental approach to estimate microtubule-generated forces through the extension of a fluorescent spring-shaped DNA origami molecule. Optical readout of the spring extension enables recording of force production simultaneously with single-molecule fluorescence of proteins getting recruited to the site of force generation. DNA nanosprings enable multiplexing of force measurements and only require a fluorescence microscope and basic laboratory equipment. We validate the performance of DNA nanosprings against results obtained using optical trapping. Finally, we demonstrate the use of the nanospring to study proteins that couple microtubule growth and shortening to force generation.

Original languageEnglish
Article numberjcs260154
Number of pages18
JournalJournal of cell science
Issue number5
Publication statusPublished - 2023

Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.


  • DNA origami
  • Dynein
  • Force sensor
  • Kinetochore
  • Microtubule
  • Optical trap

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