TY - JOUR
T1 - Exploiting Bacteriophage Proteomes
T2 - The Hidden Biotechnological Potential
AU - Santos, Sílvio B.
AU - Costa, Ana Rita
AU - Carvalho, Carla
AU - Nóbrega, Franklin L.
AU - Azeredo, Joana
N1 - Accepted Author Manuscript
PY - 2018
Y1 - 2018
N2 - Bacteriophages encode many distinct proteins for the successful infection of a bacterial host. Each protein plays a specific role in the phage replication cycle, from host recognition, through takeover of the host machinery, and up to cell lysis for progeny release. As the roles of these proteins are being revealed, more biotechnological applications can be anticipated. Phage-encoded proteins are now being explored for the control, detection, and typing of bacteria; as vehicles for drug delivery; and for vaccine development. In this review, we discuss how engineering approaches can be used to improve the natural properties of these proteins and set forth the most innovative applications that demonstrate the unlimited biotechnological potential held by phage-encoded proteins.
AB - Bacteriophages encode many distinct proteins for the successful infection of a bacterial host. Each protein plays a specific role in the phage replication cycle, from host recognition, through takeover of the host machinery, and up to cell lysis for progeny release. As the roles of these proteins are being revealed, more biotechnological applications can be anticipated. Phage-encoded proteins are now being explored for the control, detection, and typing of bacteria; as vehicles for drug delivery; and for vaccine development. In this review, we discuss how engineering approaches can be used to improve the natural properties of these proteins and set forth the most innovative applications that demonstrate the unlimited biotechnological potential held by phage-encoded proteins.
KW - anti-CRISPR
KW - bacteria control and detection
KW - bacteriolytic activity
KW - genetic engineering
KW - host specificity
KW - Phage-encoded proteins
UR - http://resolver.tudelft.nl/uuid:69e5b965-a6e8-4e7c-8579-00026bc80683
UR - http://www.scopus.com/inward/record.url?scp=85046884643&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2018.04.006
DO - 10.1016/j.tibtech.2018.04.006
M3 - Article
AN - SCOPUS:85046884643
SN - 0167-7799
JO - Trends in Biotechnology
JF - Trends in Biotechnology
ER -
