TY - JOUR
T1 - Extracellular vesicle quantification and characterization
T2 - Common methods and emerging approaches
AU - Hartjes, Thomas A.
AU - Mytnyk, Serhii
AU - Jenster, Guido W.
AU - van Steijn, Volkert
AU - van Royen, Martin E.
PY - 2019
Y1 - 2019
N2 - Extracellular vesicles (EVs) are a family of small membrane vesicles that carry information about cells by which they are secreted. Growing interest in the role of EVs in intercellular communication, but also in using their diagnostic, prognostic and therapeutic potential in (bio) medical applications, demands for accurate assessment of their biochemical and physical properties. In this review, we provide an overview of available technologies for EV analysis by describing their working principles, assessing their utility in EV research and summarising their potential and limitations. To emphasise the innovations in EV analysis, we also highlight the unique possibilities of emerging technologies with high potential for further development.
AB - Extracellular vesicles (EVs) are a family of small membrane vesicles that carry information about cells by which they are secreted. Growing interest in the role of EVs in intercellular communication, but also in using their diagnostic, prognostic and therapeutic potential in (bio) medical applications, demands for accurate assessment of their biochemical and physical properties. In this review, we provide an overview of available technologies for EV analysis by describing their working principles, assessing their utility in EV research and summarising their potential and limitations. To emphasise the innovations in EV analysis, we also highlight the unique possibilities of emerging technologies with high potential for further development.
KW - Emerging technologies
KW - Extracellular vesicles (EVs)
KW - Methods for EV analysis
UR - http://www.scopus.com/inward/record.url?scp=85061834746&partnerID=8YFLogxK
U2 - 10.3390/bioengineering6010007
DO - 10.3390/bioengineering6010007
M3 - Review article
AN - SCOPUS:85061834746
SN - 2306-5354
VL - 6
JO - Bioengineering
JF - Bioengineering
IS - 1
M1 - 7
ER -