Extreme calorie restriction in yeast retentostats induces uniform non-quiescent growth arrest

Markus M.M. Bisschops, Marijke A.H. Luttik, Anne Doerr, Peter J.T. Verheijen, Frank J. Bruggeman, Jack T. Pronk, Pascale Daran-Lapujade*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)


Non-dividing Saccharomyces cerevisiae cultures are highly relevant for fundamental and applied studies. However, cultivation conditions in which non-dividing cells retain substantial metabolic activity are lacking. Unlike stationary-phase (SP) batch cultures, the current experimental paradigm for non-dividing yeast cultures, cultivation under extreme calorie restriction (ECR) in retentostat enables non-dividing yeast cells to retain substantial metabolic activity and to prevent rapid cellular deterioration. Distribution of F-actin structures and single-cell copy numbers of specific transcripts revealed that cultivation under ECR yields highly homogeneous cultures, in contrast to SP cultures that differentiate into quiescent and non-quiescent subpopulations. Combined with previous physiological studies, these results indicate that yeast cells subjected to ECR survive in an extended G1 phase. This study demonstrates that yeast cells exposed to ECR differ from carbon-starved cells and offer a promising experimental model for studying non-dividing, metabolically active, and robust eukaryotic cells.

Original languageEnglish
Pages (from-to)231-242
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Issue number1
Publication statusPublished - 2017


  • Actin structure
  • Extreme calorie restriction
  • Heterogeneity
  • Non-dividing
  • Retentostat


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