Femtosecond Laser Fabrication of Microporous Membranes for Biological Applications

Annalisa Volpe*, F.M. Conte Capodacqua, Valeria Garzarelli, Elisabetta Primiceri, Maria Serena Chiriacò, Caterina Gaudiuso, Francesco Ferrara, Antonio Ancona

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review


The possibility of fabricating micrometric pore size membranes is gaining great interest in many applications, from studying cell signaling, to filtration. Currently, many technologies are reported to fabricate such microsystems, the choice of which depends strictly on the substrate material and on the final application. Here, we demonstrate the capability with a single femtosecond laser source and experimental setup to fabricate micromembranes both on polymeric and multilayer metallic substrate, without the need for moulds, mask, and complex facilities. In particular, the flexibility of laser drilling was exploited to obtain microfilters with pore size of 8 and 18μm in diameter,on metallic and polymeric substrate, respectively, and controlled distribution. For evaluating the possibility to use such laser-fabricated membranes into biological assay, their biocompatibility has been investigated. To this aim, as a proof of concept, we tested the two materials into viability tests.The culture of mammalian cells on these microfabricated membranes were studied showing their compatibility with cells.
Original languageEnglish
Article number1371
Number of pages13
Issue number1371
Publication statusPublished - 2022
Externally publishedYes


This research was funded by the Italian Ministry of Education, University and Research (MIUR) within the Project TITAN (PON ARS01_00906), and the project PRIN 2017 “Prostate cancer: disentangling the relationships with tumor microenvironment to better model and target tumor progression” (grant number: Prot. 20174PLLYN).


  • fs-laser
  • Micromachining
  • laser drilling
  • membrane
  • cell manipulation


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