TY - JOUR
T1 - Flow preconditioning of endothelial cells on collagen-immobilized silicone fibers enhances cell retention and antithrombotic function
AU - Salehi-Nik, Nasim
AU - Banikarimi, Seyedeh Parnian
AU - Amoabediny, Ghassem
AU - Pouran, Behdad
AU - Shokrgozar, Mohammad Ali
AU - Zandieh-Doulabi, Behrouz
AU - Klein-Nulend, Jenneke
PY - 2017
Y1 - 2017
N2 - Stability and antithrombotic functionality of endothelial cells on silicone hollow fibers (SiHFs) are critical in the development of biohybrid artificial lungs. Here we aimed to enhance endothelial cell retention and anti-thrombotic function by low (12 dyn/cm2, 24 h) fluid shear stress ("flow") preconditioning of endothelial cells seeded on collagen-immobilized SiHFs. The response of endothelial cells without preconditioning (48 h static culture) and with preconditioning (24 h static culture followed by 24 h flow preconditioning) on hollow fibers to high fluid shear stress (30 dyn/cm2, 1 h) was assessed in a parallel-plate flow chamber. Finite element (FE) modeling was used to simulate shear stress within the flow chamber. We found that collagen immobilization on hollow fibers using carbodiimide bonds provided sufficient stability to high shear stress. Flow preconditioning for 24 h before treatment with high shear stress for 1 h on collagen-immobilized hollow fibers increased cell retention (1.3-fold). The FE model showed that cell flattening due to flow preconditioning reduced maximum shear stress on cells by 32%. Flow preconditioning prior to exposure to high fluid shear stress enhanced the production of nitric oxide (1.3-fold) and prostaglandin I2 (1.2-fold). In conclusion, flow preconditioning of endothelial cells on collagen-immobilized SiHFs enhanced cell retention and antithrombotic function, which could significantly improve current biohybrid artificial lungs.
AB - Stability and antithrombotic functionality of endothelial cells on silicone hollow fibers (SiHFs) are critical in the development of biohybrid artificial lungs. Here we aimed to enhance endothelial cell retention and anti-thrombotic function by low (12 dyn/cm2, 24 h) fluid shear stress ("flow") preconditioning of endothelial cells seeded on collagen-immobilized SiHFs. The response of endothelial cells without preconditioning (48 h static culture) and with preconditioning (24 h static culture followed by 24 h flow preconditioning) on hollow fibers to high fluid shear stress (30 dyn/cm2, 1 h) was assessed in a parallel-plate flow chamber. Finite element (FE) modeling was used to simulate shear stress within the flow chamber. We found that collagen immobilization on hollow fibers using carbodiimide bonds provided sufficient stability to high shear stress. Flow preconditioning for 24 h before treatment with high shear stress for 1 h on collagen-immobilized hollow fibers increased cell retention (1.3-fold). The FE model showed that cell flattening due to flow preconditioning reduced maximum shear stress on cells by 32%. Flow preconditioning prior to exposure to high fluid shear stress enhanced the production of nitric oxide (1.3-fold) and prostaglandin I2 (1.2-fold). In conclusion, flow preconditioning of endothelial cells on collagen-immobilized SiHFs enhanced cell retention and antithrombotic function, which could significantly improve current biohybrid artificial lungs.
KW - Antithrombotic function
KW - Biohybrid artificial lung
KW - Collagen immobilization
KW - Endothelialization
KW - Finite element modeling
KW - Fluid shear stress
UR - http://www.scopus.com/inward/record.url?scp=84978483900&partnerID=8YFLogxK
U2 - 10.1111/aor.12759
DO - 10.1111/aor.12759
M3 - Article
AN - SCOPUS:84978483900
SN - 0160-564X
VL - 41
SP - 556
EP - 567
JO - Artificial Organs: replacement, recovery, and regeneration
JF - Artificial Organs: replacement, recovery, and regeneration
IS - 6
ER -