Flow preconditioning of endothelial cells on collagen-immobilized silicone fibers enhances cell retention and antithrombotic function

Nasim Salehi-Nik, Seyedeh Parnian Banikarimi, Ghassem Amoabediny, Behdad Pouran, Mohammad Ali Shokrgozar, Behrouz Zandieh-Doulabi, Jenneke Klein-Nulend

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Stability and antithrombotic functionality of endothelial cells on silicone hollow fibers (SiHFs) are critical in the development of biohybrid artificial lungs. Here we aimed to enhance endothelial cell retention and anti-thrombotic function by low (12 dyn/cm2, 24 h) fluid shear stress ("flow") preconditioning of endothelial cells seeded on collagen-immobilized SiHFs. The response of endothelial cells without preconditioning (48 h static culture) and with preconditioning (24 h static culture followed by 24 h flow preconditioning) on hollow fibers to high fluid shear stress (30 dyn/cm2, 1 h) was assessed in a parallel-plate flow chamber. Finite element (FE) modeling was used to simulate shear stress within the flow chamber. We found that collagen immobilization on hollow fibers using carbodiimide bonds provided sufficient stability to high shear stress. Flow preconditioning for 24 h before treatment with high shear stress for 1 h on collagen-immobilized hollow fibers increased cell retention (1.3-fold). The FE model showed that cell flattening due to flow preconditioning reduced maximum shear stress on cells by 32%. Flow preconditioning prior to exposure to high fluid shear stress enhanced the production of nitric oxide (1.3-fold) and prostaglandin I2 (1.2-fold). In conclusion, flow preconditioning of endothelial cells on collagen-immobilized SiHFs enhanced cell retention and antithrombotic function, which could significantly improve current biohybrid artificial lungs.

Original languageEnglish
Pages (from-to)556 - 567
JournalArtificial Organs: replacement, recovery, and regeneration
Volume41
Issue number6
DOIs
Publication statusPublished - 2017

Keywords

  • Antithrombotic function
  • Biohybrid artificial lung
  • Collagen immobilization
  • Endothelialization
  • Finite element modeling
  • Fluid shear stress

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    Salehi-Nik, N., Banikarimi, S. P., Amoabediny, G., Pouran, B., Shokrgozar, M. A., Zandieh-Doulabi, B., & Klein-Nulend, J. (2017). Flow preconditioning of endothelial cells on collagen-immobilized silicone fibers enhances cell retention and antithrombotic function. Artificial Organs: replacement, recovery, and regeneration, 41(6), 556 - 567. https://doi.org/10.1111/aor.12759