Fluorescence enhancement of fluorophores in live cells using metallic nanoparticles

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Abstract

Plasmonic enhancement of fluorescence has been challenging in in vivo imaging applications. We present a study demonstrating the plasmonic enhancement of fluorescent membrane proteins within their native physiological environment using tailored metallic nanoparticles. This work highlights two schemes to influence the distance between the emitting dipoles and the enhancing nanoparticles, namely the addition of nanoparticles in the buffer solution and the incorporation in the polymer matrix at the bottom of the cells. Incorporating biological structures native to the cellular environment offers opportunities for the optimization of in vivo fluorescence imaging methods and the detection of membrane proteins.

Original languageEnglish
Title of host publicationNeurophotonics II
EditorsTomas Cizmar, Tommaso Fellin
PublisherSPIE
Number of pages5
ISBN (Electronic)9781510673328
DOIs
Publication statusPublished - 2024
EventNeurophotonics II 2024 - Strasbourg, France
Duration: 8 Apr 20249 Apr 2024

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume13007
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceNeurophotonics II 2024
Country/TerritoryFrance
CityStrasbourg
Period8/04/249/04/24

Keywords

  • Fluorescence
  • Gold nanoparticles
  • Light microscopy
  • Membrane proteins
  • Plasmonic enhancement

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