Fluorescence Polarization Control for On-Off Switching of Single Molecules at Cryogenic Temperatures

Christiaan N. Hulleman, Maximiliaan Huisman, Robert J. Moerland, David Gruenwald, Sjoerd Stallinga, Bernd Rieger

Research output: Contribution to journalArticleScientificpeer-review

19 Downloads (Pure)


Light microscopy, allowing sub-diffraction-limited resolution, has been among the fastest developing techniques at the interface of biology, chemistry, and physics. Intriguingly no theoretical limit exists on how far the underlying measurement uncertainty can be lowered. In particular data fusion of large amounts of images can reduce the measurement error to match the reso-lution of structural methods like cryo-electron microscopy. Fluorescence, although reliant on a reporter molecule and therefore not the first choice to obtain ultraresolution structures, brings highly specific labeling of molecules in a large assembly to the table and inherently allows the detection of multiple colors, which enables the interrogation of multiple molecular species at the same time in the same sample. Here, the problems to be solved in the coming years, with the aim of higher resolution, are discussed, and what polarization depletion of fluorescence at cryogenic temperatures can contribute for fluorescence imaging of biological samples, like whole cells, is described.
Original languageEnglish
Number of pages7
Issue number9
Publication statusPublished - 2018

Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.


  • cryogenic
  • polarization
  • single molecules
  • STED
  • super-resolution


Dive into the research topics of 'Fluorescence Polarization Control for On-Off Switching of Single Molecules at Cryogenic Temperatures'. Together they form a unique fingerprint.

Cite this