Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H                         
                        2
                                                 O                         
                        2
                                                 -Dependent Biocatalytic Oxidation Reactions

Florian Tieves; Sébastien Jean Paul Willot; Morten Martinus Cornelis Harald van Schie; Marine Charlène Renée Rauch; Sabry Hamdy Hamed Younes; Wuyuan Zhang; Jia Jia Dong; Patricia Gomez de Santos; Frank Hollmann

doi:10.1002/anie.201902380

Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H ₂ O ₂ -Dependent Biocatalytic Oxidation Reactions

Florian Tieves, Sébastien Jean Paul Willot, Morten Martinus Cornelis Harald van Schie, Marine Charlène Renée Rauch, Sabry Hamdy Hamed Younes, Wuyuan Zhang, Jia Jia Dong, Patricia Gomez de Santos, Frank Hollmann^*

^*Corresponding author for this work

BT/Biocatalysis

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Abstract

An increasing number of biocatalytic oxidation reactions rely on H ₂ O ₂ as a clean oxidant. The poor robustness of most enzymes towards H ₂ O ₂ , however, necessitates more efficient systems for in situ H ₂ O ₂ generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H ₂ O ₂ -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.

Original language	English
Pages (from-to)	7873-7877
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	58
Issue number	23
DOIs	https://doi.org/10.1002/anie.201902380
Publication status	Published - 2019

Keywords

biocatalysis
formate oxidase
hydrogen peroxide
oxidation
oxyfunctionalisation

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Tieves_et_al-2019-Angewandte_Chemie_International_EditionFinal published version, 1.03 MBLicence: CC BY-NC-ND

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Tieves, F., Willot, S. J. P., van Schie, M. M. C. H., Rauch, M. C. R., Younes, S. H. H., Zhang, W., Dong, J. J., Gomez de Santos, P., & Hollmann, F. (2019). Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H ₂ O ₂ -Dependent Biocatalytic Oxidation Reactions. Angewandte Chemie - International Edition, 58(23), 7873-7877. https://doi.org/10.1002/anie.201902380

Tieves, Florian ; Willot, Sébastien Jean Paul ; van Schie, Morten Martinus Cornelis Harald et al. / Formate Oxidase (FOx) from Aspergillus oryzae : One Catalyst Enables Diverse H ₂ O ₂ -Dependent Biocatalytic Oxidation Reactions. In: Angewandte Chemie - International Edition. 2019 ; Vol. 58, No. 23. pp. 7873-7877.

@article{7cab555197fc4dfd89bd9521b7af7c5c,

title = "Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H 2 O 2 -Dependent Biocatalytic Oxidation Reactions",

abstract = " An increasing number of biocatalytic oxidation reactions rely on H 2 O 2 as a clean oxidant. The poor robustness of most enzymes towards H 2 O 2 , however, necessitates more efficient systems for in situ H 2 O 2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H 2 O 2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved. ",

keywords = "biocatalysis, formate oxidase, hydrogen peroxide, oxidation, oxyfunctionalisation",

author = "Florian Tieves and Willot, {S{\'e}bastien Jean Paul} and {van Schie}, {Morten Martinus Cornelis Harald} and Rauch, {Marine Charl{\`e}ne Ren{\'e}e} and Younes, {Sabry Hamdy Hamed} and Wuyuan Zhang and Dong, {Jia Jia} and {Gomez de Santos}, Patricia and Frank Hollmann",

year = "2019",

doi = "10.1002/anie.201902380",

language = "English",

volume = "58",

pages = "7873--7877",

journal = "Angewandte Chemie - International Edition",

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publisher = "John Wiley and Sons",

number = "23",

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Tieves, F, Willot, SJP, van Schie, MMCH, Rauch, MCR, Younes, SHH, Zhang, W, Dong, JJ, Gomez de Santos, P & Hollmann, F 2019, 'Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H ₂ O ₂ -Dependent Biocatalytic Oxidation Reactions', Angewandte Chemie - International Edition, vol. 58, no. 23, pp. 7873-7877. https://doi.org/10.1002/anie.201902380

Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H ₂ O ₂ -Dependent Biocatalytic Oxidation Reactions. / Tieves, Florian; Willot, Sébastien Jean Paul; van Schie, Morten Martinus Cornelis Harald et al.
In: Angewandte Chemie - International Edition, Vol. 58, No. 23, 2019, p. 7873-7877.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Formate Oxidase (FOx) from Aspergillus oryzae

T2 - One Catalyst Enables Diverse H 2 O 2 -Dependent Biocatalytic Oxidation Reactions

AU - Tieves, Florian

AU - Willot, Sébastien Jean Paul

AU - van Schie, Morten Martinus Cornelis Harald

AU - Rauch, Marine Charlène Renée

AU - Younes, Sabry Hamdy Hamed

AU - Zhang, Wuyuan

AU - Dong, Jia Jia

AU - Gomez de Santos, Patricia

AU - Hollmann, Frank

PY - 2019

Y1 - 2019

N2 - An increasing number of biocatalytic oxidation reactions rely on H 2 O 2 as a clean oxidant. The poor robustness of most enzymes towards H 2 O 2 , however, necessitates more efficient systems for in situ H 2 O 2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H 2 O 2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.

AB - An increasing number of biocatalytic oxidation reactions rely on H 2 O 2 as a clean oxidant. The poor robustness of most enzymes towards H 2 O 2 , however, necessitates more efficient systems for in situ H 2 O 2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H 2 O 2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.

KW - biocatalysis

KW - formate oxidase

KW - hydrogen peroxide

KW - oxidation

KW - oxyfunctionalisation

UR - http://www.scopus.com/inward/record.url?scp=85065201966&partnerID=8YFLogxK

U2 - 10.1002/anie.201902380

DO - 10.1002/anie.201902380

M3 - Article

AN - SCOPUS:85065201966

SN - 1433-7851

VL - 58

SP - 7873

EP - 7877

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 23

ER -

Tieves F, Willot SJP, van Schie MMCH, Rauch MCR, Younes SHH, Zhang W et al. Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H ₂ O ₂ -Dependent Biocatalytic Oxidation Reactions. Angewandte Chemie - International Edition. 2019;58(23):7873-7877. doi: 10.1002/anie.201902380

Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H ₂ O ₂ -Dependent Biocatalytic Oxidation Reactions

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Keywords

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