Genome duplication and mutations in ACE2 cause multicellar, fast-sedimenting phenotypes in evolved Saccharomyces cerevisiae

B Oud, VG Guadalupe Medina, K Nijkamp, Dick de Ridder, JT Pronk, AJA van Maris, JM Daran

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

54 Citations (Scopus)

Abstract

Laboratory evolution of the yeast Saccharomyces cerevisiae in bioreactor batch cultures yielded variants that grow as multicellular, fast-sedimenting clusters. Knowledge of the molecular basis of this phenomenon may contribute to the understanding of natural evolution of multicellularity and to manipulating cell sedimentation in laboratory and industrial applications of S. cerevisiae. Multicellular, fast-sedimenting lineages obtained from a haploid S. cerevisiae strain in two independent evolution experiments were analyzed by whole genome resequencing. The two evolved cell lines showed different frameshift mutations in a stretch of eight adenosines in ACE2, which encodes a transcriptional regulator involved in cell cycle control and mother-daughter cell separation. Introduction of the two ace2 mutant alleles into the haploid parental strain led to slow-sedimenting cell clusters that consisted of just a few cells, thus representing only a partial reconstruction of the evolved phenotype. In addition to single-nucleotide mutations, a whole-genome duplication event had occurred in both evolved multicellular strains. Construction of a diploid reference strain with two mutant ace2 alleles led to complete reconstruction of the multicellular-fast sedimenting phenotype. This study shows that whole-genome duplication and a frameshift mutation in ACE2 are sufficient to generate a fast-sedimenting, multicellular phenotype in S. cerevisiae. The nature of the ace2 mutations and their occurrence in two independent evolution experiments encompassing fewer than 500 generations of selective growth suggest that switching between unicellular and multicellular phenotypes may be relevant for competitiveness of S. cerevisiae in natural environments.
Original languageEnglish
Title of host publicationProceedings of The National Academy of Sciences of the United States of America
EditorsA.L. Demain
PagesE4223-E4231
Number of pages9
DOIs
Publication statusPublished - 2013
EventPYFF5 - s.l.
Duration: 4 Jun 20137 Jun 2013

Publication series

NameProceedings of the National Academy of Sciences of the United States of America
Volume110
ISSN (Print)0027-8424

Conference

ConferencePYFF5
Period4/06/137/06/13

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