TY - JOUR
T1 - Genotoxicity in the absence of inflammation after tungsten inhalation in mice
AU - Sørli, Jorid B.
AU - Jensen, Alexander C.Ø.
AU - Mortensen, Alicja
AU - Szarek, Józef
AU - Gutierrez, Claudia A.T.
AU - Hafez, Iosif
AU - Biskos, George
AU - Hougaard, Karin S.
AU - Vogel, Ulla
AU - More Authors, null
PY - 2023
Y1 - 2023
N2 - Tungsten is used in several applications and human exposure may occur. To assess its pulmonary toxicity, we exposed male mice to nose-only inhalation of tungsten particles at 9, 23 or 132 mg/m3 (Low, Mid and High exposure) (45 min/day, 5 days/week for 2 weeks). Increased genotoxicity (assessed by comet assay) was seen in bronchoalveolar (BAL) fluid cells at Low and High exposure. We measured acellular ROS production, and cannot exclude that ROS contributed to the observed genotoxicity. We saw no effects on body weight gain, pulmonary inflammation, lactate dehydrogenase or protein in BAL fluid, pathology of liver or kidney, or on sperm counts. In conclusion, tungsten showed non-dose dependent genotoxicity in the absence of inflammation and therefore interpreted to be primary genotoxicity. Based on genotoxicity, a Lowest Observed Adverse Effect Concentration (LOAEC) could be set at 9 mg/m3. It was not possible to establish a No Adverse Effect Concentration (NOAEC).
AB - Tungsten is used in several applications and human exposure may occur. To assess its pulmonary toxicity, we exposed male mice to nose-only inhalation of tungsten particles at 9, 23 or 132 mg/m3 (Low, Mid and High exposure) (45 min/day, 5 days/week for 2 weeks). Increased genotoxicity (assessed by comet assay) was seen in bronchoalveolar (BAL) fluid cells at Low and High exposure. We measured acellular ROS production, and cannot exclude that ROS contributed to the observed genotoxicity. We saw no effects on body weight gain, pulmonary inflammation, lactate dehydrogenase or protein in BAL fluid, pathology of liver or kidney, or on sperm counts. In conclusion, tungsten showed non-dose dependent genotoxicity in the absence of inflammation and therefore interpreted to be primary genotoxicity. Based on genotoxicity, a Lowest Observed Adverse Effect Concentration (LOAEC) could be set at 9 mg/m3. It was not possible to establish a No Adverse Effect Concentration (NOAEC).
KW - Comet assay
KW - Pulmonary
KW - Toxicity
KW - Toxicology
KW - Wolfram
UR - http://www.scopus.com/inward/record.url?scp=85147277179&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2023.104074
DO - 10.1016/j.etap.2023.104074
M3 - Article
C2 - 36724834
AN - SCOPUS:85147277179
SN - 1382-6689
VL - 98
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
M1 - 104074
ER -