Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes

Dimitry Y. Sorokin; Alexander G.  Elcheninov; Alexander Y. Merkel; Nocole J. Bale; Jaap S.  Sininghe-Damste; Ilya V. Kublanov

doi:10.1016/j.syapm.2023.126471

Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes

Dimitry Y. Sorokin^*, Alexander G. Elcheninov, Alexander Y. Merkel, Nocole J. Bale, Jaap S. Sininghe-Damste, Ilya V. Kublanov

^*Corresponding author for this work

BT/Environmental Biotechnology

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Abstract

Two strains of neutrophilic haloaloarchaea were selectively enriched from hypersaline lakes in southwestern Siberia using β-1,3-glucans as a substrate. The strains were nearly identical in their phenotypes and according to
phylogenomic analysis, and represent a distant novel species group in the genus Halapricum of the family Haloarculaceae.
The main phenotypic property of the novel isolates is the ability to hydrolyze and grow with the polysaccharides curdlan and pachyman. Such potential has, to date, not been seen in any other haloarchaea in pure cultures. The strains are obligately aerobic saccharolytics. Apart from the insoluble β-1,3-glucans, they utilized soluble α-glucans (starch, pullulan and glycogen) and a limited number of sugars. The major ether-bound polar phospholipids include PGP-Me and PG. The glyco- and sulfolipids were absent. The major respiratory menaquinone is MK-8:8. On the basis of their unique physiological properties and the results of phylogenomic analysis, the isolates are suggested to be classified into a novel species Halapricum hydrolyticum sp. nov. (type strain HArc-curdl5-1T = DSM 114193T = UQM 41587T).

Original language	English
Article number	126471
Number of pages	9
Journal	Systematic and Applied Microbiology
Volume	46
Issue number	6
DOIs	https://doi.org/10.1016/j.syapm.2023.126471
Publication status	Published - 2023

Keywords

Hypersaline lakes
Haloarchaea
β-1,3-glucans
Curdlan
Pachyman
Halapricum

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10.1016/j.syapm.2023.126471

1-s2.0-S0723202023000802-mainFinal published version, 3.93 MBLicence: CC BY

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title = "Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes",

abstract = "Two strains of neutrophilic haloaloarchaea were selectively enriched from hypersaline lakes in southwestern Siberia using β-1,3-glucans as a substrate. The strains were nearly identical in their phenotypes and according tophylogenomic analysis, and represent a distant novel species group in the genus Halapricum of the family Haloarculaceae.The main phenotypic property of the novel isolates is the ability to hydrolyze and grow with the polysaccharides curdlan and pachyman. Such potential has, to date, not been seen in any other haloarchaea in pure cultures. The strains are obligately aerobic saccharolytics. Apart from the insoluble β-1,3-glucans, they utilized soluble α-glucans (starch, pullulan and glycogen) and a limited number of sugars. The major ether-bound polar phospholipids include PGP-Me and PG. The glyco- and sulfolipids were absent. The major respiratory menaquinone is MK-8:8. On the basis of their unique physiological properties and the results of phylogenomic analysis, the isolates are suggested to be classified into a novel species Halapricum hydrolyticum sp. nov. (type strain HArc-curdl5-1T = DSM 114193T = UQM 41587T).",

keywords = "Hypersaline lakes, Haloarchaea, β-1,3-glucans, Curdlan, Pachyman, Halapricum",

author = "Sorokin, {Dimitry Y.} and Elcheninov, {Alexander G.} and Merkel, {Alexander Y.} and Bale, {Nocole J.} and Sininghe-Damste, {Jaap S.} and Kublanov, {Ilya V.}",

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journal = "Systematic and Applied Microbiology",

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T1 - Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes

AU - Sorokin, Dimitry Y.

AU - Elcheninov, Alexander G.

AU - Merkel, Alexander Y.

AU - Bale, Nocole J.

AU - Sininghe-Damste, Jaap S.

AU - Kublanov, Ilya V.

PY - 2023

Y1 - 2023

N2 - Two strains of neutrophilic haloaloarchaea were selectively enriched from hypersaline lakes in southwestern Siberia using β-1,3-glucans as a substrate. The strains were nearly identical in their phenotypes and according tophylogenomic analysis, and represent a distant novel species group in the genus Halapricum of the family Haloarculaceae.The main phenotypic property of the novel isolates is the ability to hydrolyze and grow with the polysaccharides curdlan and pachyman. Such potential has, to date, not been seen in any other haloarchaea in pure cultures. The strains are obligately aerobic saccharolytics. Apart from the insoluble β-1,3-glucans, they utilized soluble α-glucans (starch, pullulan and glycogen) and a limited number of sugars. The major ether-bound polar phospholipids include PGP-Me and PG. The glyco- and sulfolipids were absent. The major respiratory menaquinone is MK-8:8. On the basis of their unique physiological properties and the results of phylogenomic analysis, the isolates are suggested to be classified into a novel species Halapricum hydrolyticum sp. nov. (type strain HArc-curdl5-1T = DSM 114193T = UQM 41587T).

AB - Two strains of neutrophilic haloaloarchaea were selectively enriched from hypersaline lakes in southwestern Siberia using β-1,3-glucans as a substrate. The strains were nearly identical in their phenotypes and according tophylogenomic analysis, and represent a distant novel species group in the genus Halapricum of the family Haloarculaceae.The main phenotypic property of the novel isolates is the ability to hydrolyze and grow with the polysaccharides curdlan and pachyman. Such potential has, to date, not been seen in any other haloarchaea in pure cultures. The strains are obligately aerobic saccharolytics. Apart from the insoluble β-1,3-glucans, they utilized soluble α-glucans (starch, pullulan and glycogen) and a limited number of sugars. The major ether-bound polar phospholipids include PGP-Me and PG. The glyco- and sulfolipids were absent. The major respiratory menaquinone is MK-8:8. On the basis of their unique physiological properties and the results of phylogenomic analysis, the isolates are suggested to be classified into a novel species Halapricum hydrolyticum sp. nov. (type strain HArc-curdl5-1T = DSM 114193T = UQM 41587T).

KW - Hypersaline lakes

KW - Haloarchaea

KW - β-1,3-glucans

KW - Curdlan

KW - Pachyman

KW - Halapricum

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U2 - 10.1016/j.syapm.2023.126471

DO - 10.1016/j.syapm.2023.126471

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SN - 0723-2020

VL - 46

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

IS - 6

M1 - 126471

ER -

Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes

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Corrigendum to “Halapricum hydrolyticum sp. nov., a beta-1,3-glucan utilizing haloarchaeon from hypersaline lakes” [Syst. Appl. Microbiol. 46(6) (2023) 126471](S0723202023000309)(10.1016/j.syapm.2023.126421)

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