High speed wavefront sensorless aberration correction in digital micromirror based confocal microscopy

Paolo Pozzi, Dean Wilding, Oleg Soloviev, Hans Verstraete, Laurens Bliek, Gleb Vdovine, Michel Verhaegen

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)


The quality of fluorescence microscopy images is often impaired by the presence
of sample induced optical aberrations. Adaptive optical elements such as deformable mirrors or spatial light modulators can be used to correct aberrations. However, previously reported techniques either require special sample preparation, or time consuming optimization procedures for the correction of static aberrations. This paper reports a technique for optical sectioning fluorescence microscopy capable of correcting dynamic aberrations in any fluorescent sample during the acquisition. This is achieved by implementing adaptive optics in a non conventional confocal microscopy setup, with multiple programmable confocal apertures, in which out of focus light can be separately detected, and used to optimize the correction performance with a sampling frequency an order of magnitude faster than the imaging rate of the system. The
paper reports results comparing the correction performances to traditional image optimization algorithms, and demonstrates how the system can compensate for dynamic changes in the aberrations, such as those introduced during a focal stack acquisition though a thick sample.
Original languageEnglish
Pages (from-to)949-959
JournalOptics Express
Issue number2
Publication statusPublished - 2017


Dive into the research topics of 'High speed wavefront sensorless aberration correction in digital micromirror based confocal microscopy'. Together they form a unique fingerprint.

Cite this