TY - JOUR
T1 - High-throughput data-driven analysis of myofiber composition reveals muscle-specific disease and age-associated patterns
AU - Raz, Vered
AU - Raz, Yotam
AU - van de Vijver, Davy
AU - Bindellini, Davide
AU - van Putten, Maaike
AU - van den Akker, Erik
PY - 2019
Y1 - 2019
N2 - Contractile properties of myofibers are dictated by the abundance of myosin heavy chain (MyHC) isoforms. MyHC composition designates muscle function, and its alterations could unravel differential muscle involvement in muscular dystrophies and aging. Current analyses are limited to visual assessments in which myofibers expressing multiple MyHC isoforms are prone to misclassification. As a result, complex patterns and subtle alterations are unidentified. We developed a high-throughput, data-driven myofiber analysis to quantitatively describe the variations in myofibers across the muscle. We investigated alterations in myofiber composition between genotypes, 2 muscles, and 2 age groups. We show that this analysis facilitates the discovery of complex myofiber compositions and its dependency on age, muscle type, and genetic conditions.—Raz, V., Raz, Y., van de Vijver, D., Bindellini, D., van Putten, M., van den Akker, E. B. High-throughput data-driven analysis of myofiber composition reveals muscle-specific disease and age-associated patterns. FASEB J. 33, 4046–4053 (2019). www.fasebj.org.
AB - Contractile properties of myofibers are dictated by the abundance of myosin heavy chain (MyHC) isoforms. MyHC composition designates muscle function, and its alterations could unravel differential muscle involvement in muscular dystrophies and aging. Current analyses are limited to visual assessments in which myofibers expressing multiple MyHC isoforms are prone to misclassification. As a result, complex patterns and subtle alterations are unidentified. We developed a high-throughput, data-driven myofiber analysis to quantitatively describe the variations in myofibers across the muscle. We investigated alterations in myofiber composition between genotypes, 2 muscles, and 2 age groups. We show that this analysis facilitates the discovery of complex myofiber compositions and its dependency on age, muscle type, and genetic conditions.—Raz, V., Raz, Y., van de Vijver, D., Bindellini, D., van Putten, M., van den Akker, E. B. High-throughput data-driven analysis of myofiber composition reveals muscle-specific disease and age-associated patterns. FASEB J. 33, 4046–4053 (2019). www.fasebj.org.
KW - ageing
KW - limb girdle muscular dystrophy
KW - myosin heavy chain
KW - SGCA-null
KW - SGCD-null
UR - http://www.scopus.com/inward/record.url?scp=85079754891&partnerID=8YFLogxK
U2 - 10.1096/fj.201801714R
DO - 10.1096/fj.201801714R
M3 - Article
C2 - 30485132
AN - SCOPUS:85079754891
SN - 0892-6638
VL - 33
SP - 4046
EP - 4053
JO - FASEB Journal
JF - FASEB Journal
IS - 3
ER -