High-throughput microdroplet-based analysis of post-translational protein modifications using mass spectrometry

Simon K. Küster, Martin Pabst, Renato Zenobi, Petra S. Dittrich

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

Abstract

We present a novel microdroplet-based microarray interface between nano-LC and MALDI-MS and demonstrate its versatility for the reliable identification of post-translational protein modifications. A tryptic protein digest containing phosphorylated and glycosylated peptides is separated by a standard nano-LC run, the eluate is compartmentalized into microdroplets and spotted onto a microarray plate, thereby conserving the chromatographic separation. A single eluting peak is split over multiple sample spots. Specific enzymes are applied to every second sample spot to remove the modification, which results in a mass shift for modified peptides. Due to this, a comparison between two consecutive MS spectra allows a robust identification of peptides carrying post-translational modifications.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1341-1343
ISBN (Print)9781632666246
Publication statusPublished - 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • Droplet microfluidics
  • Glycosylation
  • Liquid chromatography
  • MALDI-MS
  • Mass spectrometry
  • Microarrays
  • Phosphorylation
  • Post-translational modifications
  • Proteomics

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