@inproceedings{b4d4b196ef944b9d999ae85236c36485,
title = "High-throughput microdroplet-based analysis of post-translational protein modifications using mass spectrometry",
abstract = "We present a novel microdroplet-based microarray interface between nano-LC and MALDI-MS and demonstrate its versatility for the reliable identification of post-translational protein modifications. A tryptic protein digest containing phosphorylated and glycosylated peptides is separated by a standard nano-LC run, the eluate is compartmentalized into microdroplets and spotted onto a microarray plate, thereby conserving the chromatographic separation. A single eluting peak is split over multiple sample spots. Specific enzymes are applied to every second sample spot to remove the modification, which results in a mass shift for modified peptides. Due to this, a comparison between two consecutive MS spectra allows a robust identification of peptides carrying post-translational modifications.",
keywords = "Droplet microfluidics, Glycosylation, Liquid chromatography, MALDI-MS, Mass spectrometry, Microarrays, Phosphorylation, Post-translational modifications, Proteomics",
author = "K{\"u}ster, {Simon K.} and Martin Pabst and Renato Zenobi and Dittrich, {Petra S.}",
year = "2013",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "1341--1343",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}