TY - JOUR
T1 - High throughput process development for the purification of rapeseed proteins napin and cruciferin by ion exchange chromatography
AU - Moreno-González, Mónica
AU - Chuekitkumchorn, Pattra
AU - Silva, Marcelo
AU - Groenewoud, Roos
AU - Ottens, Marcel
PY - 2021
Y1 - 2021
N2 - Proteins derived from plant resources such as oilseed meals, canola and sunflower, are considered a viable alternative to animal proteins for food consumption. This work presents a rational methodology, using high throughput experimentation (HTE), for the separation of cruciferin and napin, the two major proteins of canola meal, by chromatography. Eight different mixed mode and ion exchange resins were evaluated at different conditions with the aim of capturing napin and identifying adsorption/desorption behavior, ease of desorption and selectivity. POROS 50 HS resulted as the most promising resin. The obtained equilibrium adsorption data for napin and cruciferin was used in a mechanistic chromatography model and compared with experimental results showing a very good agreement. The model was used to identify column operating parameters that lead to >98% yield and purity for both proteins. Subsequently a conceptual downstream processing was proposed.
AB - Proteins derived from plant resources such as oilseed meals, canola and sunflower, are considered a viable alternative to animal proteins for food consumption. This work presents a rational methodology, using high throughput experimentation (HTE), for the separation of cruciferin and napin, the two major proteins of canola meal, by chromatography. Eight different mixed mode and ion exchange resins were evaluated at different conditions with the aim of capturing napin and identifying adsorption/desorption behavior, ease of desorption and selectivity. POROS 50 HS resulted as the most promising resin. The obtained equilibrium adsorption data for napin and cruciferin was used in a mechanistic chromatography model and compared with experimental results showing a very good agreement. The model was used to identify column operating parameters that lead to >98% yield and purity for both proteins. Subsequently a conceptual downstream processing was proposed.
KW - Canola meal proteins
KW - Chromatography
KW - Column simulation
KW - High throughput experimentation
KW - Ion Exchange
UR - http://www.scopus.com/inward/record.url?scp=85098461327&partnerID=8YFLogxK
U2 - 10.1016/j.fbp.2020.11.011
DO - 10.1016/j.fbp.2020.11.011
M3 - Article
AN - SCOPUS:85098461327
SN - 0960-3085
VL - 125
SP - 228
EP - 241
JO - Food and Bioproducts Processing
JF - Food and Bioproducts Processing
ER -