Abstract
We present a novel design of elastic micropillars for tissue self-assembly in engineered heart tissue (EHT) platforms. The innovative tapered profile confines reproducibly the tissue position along the main micropillar axis, increasing the accuracy of tissue contraction force measurement. Polydimethylsiloxane-based pillars were designed and fabricated by wafer-level molding in an hourglass shape, with symmetric tapering producing a restriction for tissue movement in the middle of the pillars’ length. Confinement efficacy of the new geometry was validated by comparing the tissue performance in straight versus tapered (75° or 80° tapering angle) micropillars. While in all three cases compact tissues formed successfully, for both tapered designs the functionality assays evidenced yield increase from 15% to 100%, higher spatial tissue confinement, and correspondingly higher accuracy and smaller dispersion in measurements of tissue contraction force.
Original language | English |
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Title of host publication | Proceedings of the 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS) |
Place of Publication | Danvers |
Publisher | IEEE |
Pages | 374-377 |
Number of pages | 4 |
ISBN (Electronic) | 978-1-6654-9308-6 |
ISBN (Print) | 978-1-6654-9309-3 |
DOIs | |
Publication status | Published - 2023 |
Event | 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS) - Munich, Germany Duration: 15 Jan 2023 → 19 Jan 2023 Conference number: 36th |
Conference
Conference | 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS) |
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Country/Territory | Germany |
City | Munich |
Period | 15/01/23 → 19/01/23 |
Bibliographical note
Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-careOtherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
Keywords
- engineered heart tissue
- heart-on-chip
- organ-on-chip
- microfabrication