Highly reproducible tissue positioning with tapered pillar design in engineered heart tissue platforms

Milica Dostanic, Laura Windt, Maury Wiendels, Berend J. van Meer, Christine L. Mummery, Pasqualina M. Sarro, Massimo Mastrangeli

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

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We present a novel design of elastic micropillars for tissue self-assembly in engineered heart tissue (EHT) platforms. The innovative tapered profile confines reproducibly the tissue position along the main micropillar axis, increasing the accuracy of tissue contraction force measurement. Polydimethylsiloxane-based pillars were designed and fabricated by wafer-level molding in an hourglass shape, with symmetric tapering producing a restriction for tissue movement in the middle of the pillars’ length. Confinement efficacy of the new geometry was validated by comparing the tissue performance in straight versus tapered (75° or 80° tapering angle) micropillars. While in all three cases compact tissues formed successfully, for both tapered designs the functionality assays evidenced yield increase from 15% to 100%, higher spatial tissue confinement, and correspondingly higher accuracy and smaller dispersion in measurements of tissue contraction force.
Original languageEnglish
Title of host publicationProceedings of the 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS)
Place of PublicationDanvers
Number of pages4
ISBN (Electronic)978-1-6654-9308-6
ISBN (Print)978-1-6654-9309-3
Publication statusPublished - 2023
Event2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS) - Munich, Germany
Duration: 15 Jan 202319 Jan 2023
Conference number: 36th


Conference2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS)

Bibliographical note

Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care
Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.


  • engineered heart tissue
  • heart-on-chip
  • organ-on-chip
  • microfabrication

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