Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples

Guinevere S.M. Lageveen-Kammeijer, Noortje de Haan, Pablo Mohaupt, Sander Wagt, Mike Filius, Jan Nouta, David Falck, Manfred Wuhrer

Research output: Contribution to journalArticleScientificpeer-review

30 Citations (Scopus)
76 Downloads (Pure)

Abstract

The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery. Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts. Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans. This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.

Original languageEnglish
Article number2137
Number of pages8
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019

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