Homeostasis in intestinal organoids at the single cell level

How does the small intestine maintain itself? What mechanism does it use to achieve homeostasis, and how optimal is this mechanism? We approached these questions mainly using cell tracking in organoids, for which we developed new cell trackers. We found that the intestinal crypt uses a surprisingly simple and effective strategy. Finally, we showed that cell segmentation and tracking is possible in organoids without fluorescent markers.