How innovations in methodology offer new prospects for volume electron microscopy

Arent J. Kievits*, Ryan Lane, Elizabeth C. Carroll, Jacob P. Hoogenboom

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

6 Citations (Scopus)
107 Downloads (Pure)

Abstract

Detailed knowledge of biological structure has been key in understanding biology at several levels of organisation, from organs to cells and proteins. Volume electron microscopy (volume EM) provides high resolution 3D structural information about tissues on the nanometre scale. However, the throughput rate of conventional electron microscopes has limited the volume size and number of samples that can be imaged. Recent improvements in methodology are currently driving a revolution in volume EM, making possible the structural imaging of whole organs and small organisms. In turn, these recent developments in image acquisition have created or stressed bottlenecks in other parts of the pipeline, like sample preparation, image analysis and data management. While the progress in image analysis is stunning due to the advent of automatic segmentation and server-based annotation tools, several challenges remain. Here we discuss recent trends in volume EM, emerging methods for increasing throughput and implications for sample preparation, image analysis and data management.

Original languageEnglish
Pages (from-to)114-137
Number of pages24
JournalJournal of Microscopy
Volume287
Issue number3
DOIs
Publication statusPublished - 2022

Keywords

  • data management
  • image analysis
  • image processing
  • MB-SEM
  • methodology development
  • volume EM

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