TY - JOUR
T1 - Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs
AU - Ticar, Bernadeth F.
AU - Rohmah, Zuliyati
AU - Mussatto, Solange I.
AU - Lim, Jae Min
AU - Park, Seongha
AU - Choi, Byeong Dae
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3 > F2 > F1 > Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The electrospray ionization tandem mass spectrometry (ESI-MS/MS) confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among non-methylated, sulfated disaccharides.
AB - Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3 > F2 > F1 > Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The electrospray ionization tandem mass spectrometry (ESI-MS/MS) confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among non-methylated, sulfated disaccharides.
KW - Hyaluronic acid
KW - Hyaluronidase inhibitory effect
KW - L. tessellatus eggs
UR - http://www.scopus.com/inward/record.url?scp=85008157871&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2016.12.065
DO - 10.1016/j.carbpol.2016.12.065
M3 - Article
AN - SCOPUS:85008157871
SN - 0144-8617
VL - 161
SP - 16
EP - 20
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -