Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Bernadeth F. Ticar, Zuliyati Rohmah, Solange I. Mussatto, Jae Min Lim, Seongha Park, Byeong Dae Choi

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3 > F2 > F1 > Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The electrospray ionization tandem mass spectrometry (ESI-MS/MS) confirmed the presence of uronic acids on F3, which could be a 0,2A2 fragment plus loss of methyl group which is very common among non-methylated, sulfated disaccharides.

Original languageEnglish
Pages (from-to)16-20
Number of pages5
JournalCarbohydrate Polymers
Volume161
DOIs
Publication statusPublished - 1 Apr 2017
Externally publishedYes

Keywords

  • Hyaluronic acid
  • Hyaluronidase inhibitory effect
  • L. tessellatus eggs

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