Hybrid adaptive and computational light-sheet fluorescence microscopy

Dean Wilding, Paolo Pozzi, Oleg Soloviev, Gleb Vdovin, Reto Fiolka, Michel Verhaegen

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

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Abstract

The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path providing the optical sectioning and the fluorescence detection path are both affected by the aberrations in different ways. To overcome these difficulties, we have developed hybrid adaptive optical and computational microscopy techniques to remove the effect of the aberrations in both the excitation and the fluorescence paths of these microscopes.

Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationAdaptive Optics and Wavefront Control for Biological Systems IV
EditorsT.G. Bifano, J. Kubby, S. Gigan
Place of PublicationBellingham, WA, USA
PublisherSPIE
Number of pages8
Volume10502
ISBN (Electronic)9781510614895
DOIs
Publication statusPublished - 2018
EventSPIE BIOS: Adaptive Optics and Wavefront Control for Biological Systems IV 2018 - San Francisco, United States
Duration: 27 Jan 201829 Jan 2018

Publication series

NameProceedings of SPIE
Volume10502

Conference

ConferenceSPIE BIOS
CountryUnited States
CitySan Francisco
Period27/01/1829/01/18

Keywords

  • Adaptive optics
  • deconvolution
  • light-sheet
  • microscopy

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