ImaCytE: Visual Exploration of Cellular Micro-Environments for Imaging Mass Cytometry Data

Antonios Somarakis, Vincent van Unen, Frits Koning, Boudewijn Lelieveldt, Thomas Hollt

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

Tissue functionality is determined by the characteristics of tissue-resident cells and their interactions within their microenvironment. Imaging Mass Cytometry offers the opportunity to distinguish cell types with high precision and link them to their spatial location in intact tissues at sub-cellular resolution. This technology produces large amounts of spatially-resolved high-dimensional data, which constitutes a serious challenge for the data analysis. We present an interactive visual analysis workflow for the end-to-end analysis of Imaging Mass Cytometry data that was developed in close collaboration with domain expert partners. We implemented the presented workflow in an interactive visual analysis tool; ImaCytE. Our workflow is designed to allow the user to discriminate cell types according to their protein expression profiles and analyze their cellular microenvironments, aiding in the formulation or verification of hypotheses on tissue architecture and function. Finally, we show the effectiveness of our workflow and ImaCytE through a case study performed by a collaborating specialist.
Original languageEnglish
Article number8778742
Pages (from-to)98-110
Number of pages13
JournalIEEE Transactions on Visualization and Computer Graphics
Volume27
Issue number1
DOIs
Publication statusPublished - 1 Jan 2021

Keywords

  • Visual analytics
  • high-dimensional images
  • imaging mass cytometry
  • spatial omics data

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