Incorporation of Fe@Au nanoparticles into multiresponsive pNIPAM-AAc colloidal gels modulates drug uptake and release

Sulalit Bandyopadhyay, Marte Kee Andersen, Muhammad Awais Ashfaq Alvi, Anuvansh Sharma, Rajesh Raju, Birgitte H. McDonagh, Wilhelm Robert Glomm

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

Here, a synthetic method has been optimized for the synthesis of thermoresponsive and pH-responsive poly(N-isopropylacrylamide-co-acrylic acid) nanogels which are subsequently loaded with cytochrome C by using a modified breathing-in mechanism. Physico-chemical properties mapped by using dynamic light scattering (DLS) and differential scanning calorimetry (DSC) confirm the swelling/deswelling kinetics as reversible with a volume phase transition temperature (VPTT) of ~39 °C. Fe@Au nanoparticles were incorporated inside the nanogel networks by using two different methods: coating and in situ growth. The latter bears closer resemblance to the nanogels only, while the former follows the trend of bare Fe@Au nanoparticles. High loading (~96 %) and encapsulation (500 μg/mg of nanogels) of cytochrome C were obtained. Release experiments performed by using a dialysis set-up and monitored by using UV-vis spectroscopy show the highest release at 40 °C and pH 3.2 (high temperature, low pH), with maximum release from the Fe@Au-coated nanogels that also show a reverse swelling-collapse trend. The location of the drug, the incorporation and presence of Fe@Au nanoparticles and the drug incorporation method are found to control both the drug release mechanism and kinetics. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1929-1942
Number of pages14
JournalColloid and Polymer Science: Kolloid-Zeitschrift und Zeitschrift für Polymere
Volume294
Issue number12
DOIs
Publication statusPublished - 1 Dec 2016
Externally publishedYes

Keywords

  • Breathing-in
  • Core-shell nanoparticles
  • Nanogels
  • Programmed drug release
  • Volume phase transition temperature

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