TY - JOUR
T1 - Integration of mass cytometry and mass spectrometry imaging for spatially resolved single-cell metabolic profiling
AU - Nunes, Joana B.
AU - Ijsselsteijn, Marieke E.
AU - Abdelaal, Tamim
AU - Ursem, Rick
AU - van der Ploeg, Manon
AU - Giera, Martin
AU - Everts, Bart
AU - Mahfouz, Ahmed
AU - Heijs, Bram
AU - de Miranda, Noel F.C.C.
PY - 2024
Y1 - 2024
N2 - The integration of spatial omics technologies can provide important insights into the biology of tissues. Here we combined mass spectrometry imaging-based metabolomics and imaging mass cytometry-based immunophenotyping on a single tissue section to reveal metabolic heterogeneity at single-cell resolution within tissues and its association with specific cell populations such as cancer cells or immune cells. This approach has the potential to greatly increase our understanding of tissue-level interplay between metabolic processes and their cellular components.
AB - The integration of spatial omics technologies can provide important insights into the biology of tissues. Here we combined mass spectrometry imaging-based metabolomics and imaging mass cytometry-based immunophenotyping on a single tissue section to reveal metabolic heterogeneity at single-cell resolution within tissues and its association with specific cell populations such as cancer cells or immune cells. This approach has the potential to greatly increase our understanding of tissue-level interplay between metabolic processes and their cellular components.
UR - http://www.scopus.com/inward/record.url?scp=85202616297&partnerID=8YFLogxK
U2 - 10.1038/s41592-024-02392-6
DO - 10.1038/s41592-024-02392-6
M3 - Article
AN - SCOPUS:85202616297
SN - 1548-7091
VL - 21
SP - 1796
EP - 1800
JO - Nature Methods
JF - Nature Methods
IS - 10
ER -
