Interfacial protein-protein displacement at fluid interfaces

Emma B.A. Hinderink*, Marcel B.J. Meinders, Reinhard Miller, Leonard Sagis, Karin Schroën, Claire C. Berton-Carabin

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

10 Citations (Scopus)

Abstract

Protein blends are used to stabilise many traditional and emerging emulsion products, resulting in complex, non-equilibrated interfacial structures. The interface composition just after emulsification is dependent on the competitive adsorption between proteins. Over time, non-adsorbed proteins are capable of displacing the initially adsorbed ones. Such rearrangements are important to consider, since the integrity of the interfacial film could be compromised after partial displacement, which may result in the physical destabilisation of emulsions. In the present review, we critically describe various experimental techniques to assess the interfacial composition, properties and mechanisms of protein displacement. The type of information that can be obtained from the different techniques is described, from which we comment on their suitability for displacement studies. Comparative studies between model interfaces and emulsions allow for evaluating the impact of minor components and the different fluid dynamics during interface formation. We extensively discuss available mechanistic physical models that describe interfacial properties and the dynamics of complex mixed systems, with a focus on protein in-plane and bulk-interface interactions. The potential of Brownian dynamic simulations to describe the parameters that govern interfacial displacement is also addressed. This review thus provides ample information for characterising the interfacial properties over time in protein blend-stabilised emulsions, based on both experimental and modelling approaches.

Original languageEnglish
Article number102691
JournalAdvances in Colloid and Interface Science
Volume305
DOIs
Publication statusPublished - 2022
Externally publishedYes

Keywords

  • Adsorption model
  • Brownian dynamics simulations
  • Competitive adsorption
  • Interfacial rheology
  • Protein-stabilised emulsion
  • Surface equation of state

Fingerprint

Dive into the research topics of 'Interfacial protein-protein displacement at fluid interfaces'. Together they form a unique fingerprint.

Cite this