Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay

Jae Jin Lee, Sung Hyun Kim, Keon Ah Lee, Kimleng Chuon, Kwang Hwan Jung, Doseok Kim*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

DNA cyclization assay together with single-molecule FRET was employed to monitor protein-mediated bending of a short dsDNA (~ 100 bp). This method provides a simple and easy way to monitor the structural change of DNA in real-time without necessitating prior knowledge of the molecular structures for the optimal dye-labeling. This assay was applied to study how Anabaena sensory rhodopsin transducer (ASRT) facilitates loop formation of DNA as a possible mechanism for gene regulation. The ASRT-induced DNA looping was maximized at 50 mM of Na+, while Mg2+ also played an essential role in the loop formation.

Original languageEnglish
Article number23721
Number of pages10
JournalScientific Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 2021

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