Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering

Arnica Karuna, Francesco Masia, Marie Wiltshire, Rachel Errington, Paola Borri, Wolfgang Langbein

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    Abstract

    Quantitative determination of the chemical composition of unstained samples, non-invasively, with high three- dimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution.

    Original languageEnglish
    Title of host publicationProceedings of SPIE
    Subtitle of host publicationMultiphoton Microscopy in the Biomedical Sciences XIX
    EditorsAmmasi Periasamy, Peter T. C. So, Karsten Konig
    PublisherSPIE
    Number of pages11
    Volume10882
    ISBN (Electronic)978-151062406-1
    DOIs
    Publication statusPublished - 2019
    EventMultiphoton Microscopy in the Biomedical Sciences XIX 2019 - San Francisco, United States
    Duration: 3 Feb 20196 Feb 2019

    Publication series

    NameMULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIX
    ISSN (Print)0277-786X

    Conference

    ConferenceMultiphoton Microscopy in the Biomedical Sciences XIX 2019
    CountryUnited States
    CitySan Francisco
    Period3/02/196/02/19

    Keywords

    • CARS, label-free imaging
    • Coherent anti-Stokes Raman scattering microscopy
    • Coherent Raman scattering

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  • Cite this

    Karuna, A., Masia, F., Wiltshire, M., Errington, R., Borri, P., & Langbein, W. (2019). Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering. In A. Periasamy, P. T. C. So, & K. Konig (Eds.), Proceedings of SPIE: Multiphoton Microscopy in the Biomedical Sciences XIX (Vol. 10882). [108821P] (MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIX). SPIE. https://doi.org/10.1117/12.2510277