Light-sheet optimization for microscopy

Dean Wilding, Paolo Pozzi, Oleg Soloviev, Gleb Vdovine, Michel Verhaegen

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

1 Citation (Scopus)
39 Downloads (Pure)


Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section.
Original languageEnglish
Title of host publicationProceeedings of SPIE
Subtitle of host publicationAdaptive Optics and Wavefront Control for Biological Systems II
EditorsThomas G. Bifano, Joel Kubby, Sylvain Gigan
Place of PublicationBellingham, WA, USA
Number of pages8
ISBN (Print)978-1-628419511
Publication statusPublished - 2016
EventAdaptive Optics and Wavefront Control for Biological Systems II - San Francisco, United States
Duration: 13 Feb 201615 Feb 2016

Publication series

NameProceedings of SPIE
ISSN (Electronic)1605-7422


ConferenceAdaptive Optics and Wavefront Control for Biological Systems II
Country/TerritoryUnited States
CitySan Francisco


  • Adaptive optics
  • imaging
  • microscopy
  • light-sheet microscopy
  • optimization


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