@inproceedings{0d3a06953eb64da3a341e022df2c8629,
title = "Light-sheet optimization for microscopy",
abstract = "Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section. ",
keywords = "Adaptive optics, imaging, microscopy, light-sheet microscopy, optimization",
author = "Dean Wilding and Paolo Pozzi and Oleg Soloviev and Gleb Vdovine and Michel Verhaegen",
year = "2016",
doi = "10.1117/12.2210912",
language = "English",
isbn = "978-1-628419511",
volume = "9717",
series = "Proceedings of SPIE",
publisher = "SPIE",
editor = "Bifano, {Thomas G.} and Joel Kubby and Sylvain Gigan",
booktitle = "Proceeedings of SPIE",
address = "United States",
note = "Adaptive Optics and Wavefront Control for Biological Systems II ; Conference date: 13-02-2016 Through 15-02-2016",
}