Localization microscopy at doubled precision with patterned illumination

Jelmer Cnossen; Taylor Hinsdale; Rasmus Thorsen; Marijn Siemons; Florian Schueder; Ralf Jungmann; Carlas S. Smith; Bernd Rieger; Sjoerd Stallinga

doi:10.1038/s41592-019-0657-7

Localization microscopy at doubled precision with patterned illumination

Jelmer Cnossen, Taylor Hinsdale, Rasmus Thorsen, Marijn Siemons, Florian Schueder, Ralf Jungmann, Carlas S. Smith, Bernd Rieger, Sjoerd Stallinga

Research output: Contribution to journal › Article › Scientific › peer-review

7 Citations (Scopus)

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Abstract

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

Original language	English
Pages (from-to)	59-63
Journal	Nature Methods
Volume	17
Issue number	1
DOIs	https://doi.org/10.1038/s41592-019-0657-7
Publication status	Published - 2020

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Localization microscopy at doubled precision with patterned illumination

Cnossen, J. P. (Creator), Hinsdale, T. A. (Creator), Thorsen, R. Ø. (Creator), Siemons, M. (Creator), Schueder, F. (Creator), Jungmann, R. (Creator), Smith, C. S. (Creator), Rieger, B. (Creator) & Stallinga, S. (Creator), TU Delft - 4TU Centre for research data, 2019

DOI: 10.4121/UUID:B1078E64-48D5-4F42-A1A8-3386ED14D4C7

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Cite this

Cnossen, J., Hinsdale, T., Thorsen, R., Siemons, M., Schueder, F., Jungmann, R., Smith, C. S., Rieger, B., & Stallinga, S. (2020). Localization microscopy at doubled precision with patterned illumination. Nature Methods, 17(1), 59-63. https://doi.org/10.1038/s41592-019-0657-7

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AU - Smith, Carlas S.

AU - Rieger, Bernd

AU - Stallinga, Sjoerd

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