Localization microscopy at doubled precision with patterned illumination

Jelmer Cnossen; Taylor Hinsdale; Rasmus Thorsen; Marijn Siemons; Florian Schueder; Ralf Jungmann; Carlas S. Smith; Bernd Rieger; Sjoerd Stallinga

doi:10.1038/s41592-019-0657-7

Localization microscopy at doubled precision with patterned illumination

Jelmer Cnossen, Taylor Hinsdale, Rasmus Thorsen, Marijn Siemons, Florian Schueder, Ralf Jungmann, Carlas S. Smith^*, Bernd Rieger, Sjoerd Stallinga

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

Original language	English
Pages (from-to)	59-63
Journal	Nature Methods
Volume	17
Issue number	1
DOIs	https://doi.org/10.1038/s41592-019-0657-7
Publication status	Published - 2020

Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

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Cite this

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title = "Localization microscopy at doubled precision with patterned illumination",

abstract = "MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.",

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AU - Cnossen, Jelmer

AU - Hinsdale, Taylor

AU - Thorsen, Rasmus

AU - Siemons, Marijn

AU - Schueder, Florian

AU - Jungmann, Ralf

AU - Smith, Carlas S.

AU - Rieger, Bernd

AU - Stallinga, Sjoerd

N1 - Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

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N2 - MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

AB - MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

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Localization microscopy at doubled precision with patterned illumination

Abstract

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