MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.
Bibliographical noteGreen Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
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Cnossen, J. P. (Creator), Hinsdale, T. (Creator), Thorsen, R. Ø. (Creator), Siemons, M. (Creator), Schueder, F. (Creator), Jungmann, R. (Creator), Smith, C. S. (Creator), Rieger, B. (Creator) & Stallinga, S. (Creator), TU Delft - 4TU.ResearchData, 2019