Localization microscopy at doubled precision with patterned illumination

Jelmer Cnossen, Taylor Hinsdale, Rasmus Thorsen, Marijn Siemons, Florian Schueder, Ralf Jungmann, Carlas S. Smith, Bernd Rieger, Sjoerd Stallinga

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)
11 Downloads (Pure)


MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

Original languageEnglish
Pages (from-to)59-63
JournalNature Methods
Issue number1
Publication statusPublished - 2020

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