MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.
Cnossen, J. P. (Creator), Hinsdale, T. A. (Creator), Thorsen, R. Ø. (Creator), Siemons, M. (Creator), Schueder, F. (Creator), Jungmann, R. (Creator), Smith, C. S. (Creator), Rieger, B. (Creator) & Stallinga, S. (Creator), TU Delft - 4TU Centre for research data, 2019