Localization microscopy at doubled precision with patterned illumination

Jelmer Cnossen, Taylor Hinsdale, Rasmus Thorsen, Marijn Siemons, Florian Schueder, Ralf Jungmann, Carlas S. Smith, Bernd Rieger, Sjoerd Stallinga

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)
10 Downloads (Pure)

Abstract

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

Original languageEnglish
Pages (from-to)59-63
JournalNature Methods
Volume17
Issue number1
DOIs
Publication statusPublished - 2020

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    Localization microscopy at doubled precision with patterned illumination

    Cnossen, J. P. (Creator), Hinsdale, T. A. (Creator), Thorsen, R. Ø. (Creator), Siemons, M. (Creator), Schueder, F. (Creator), Jungmann, R. (Creator), Smith, C. S. (Creator), Rieger, B. (Creator) & Stallinga, S. (Creator), TU Delft - 4TU Centre for research data, 2019

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