Localization microscopy at doubled precision with patterned illumination

Jelmer Cnossen, Taylor Hinsdale, Rasmus Thorsen, Marijn Siemons, Florian Schueder, Ralf Jungmann, Carlas S. Smith*, Bernd Rieger, Sjoerd Stallinga

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

85 Citations (Scopus)
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MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

Original languageEnglish
Pages (from-to)59-63
JournalNature Methods
Issue number1
Publication statusPublished - 2020

Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.


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