Loss of gas from echogenic liposomes exposed to pulsed ultrasound

Jason L. Raymond, Ying Luan, Tao Peng, Shao Ling Huang, David D. McPherson, Michel Versluis, Nico De Jong, Christy K. Holland

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)


The destruction of echogenic liposomes (ELIP) in response to pulsed ultrasound excitations has been studied acoustically previously. However, the mechanism underlying the loss of echogenicity due to cavitation nucleated by ELIP has not been fully clarified. In this study, an ultra-high speed imaging approach was employed to observe the destruction phenomena of single ELIP exposed to ultrasound bursts at a center frequency of 6 MHz. We observed a rapid size reduction during the ultrasound excitation in 139 out of 397 (35%) ultra-high- speed recordings. The shell dilation rate, which is defined as the microbubble wall velocity divided by the instantaneous radius, Ṙ/R, was extracted from the radius versus time response of each ELIP, and was found to be correlated with the deflation. Fragmentation and surface mode vibrations were also observed and are shown to depend on the applied acoustic pressure and initial radius. Results from this study can be utilized to optimize the theranostic application of ELIP, e.g. by tuning the size distribution or the excitation frequency.

Original languageEnglish
Pages (from-to)8321-8339
JournalPhysics in Medicine and Biology
Issue number23
Publication statusPublished - 2016
Externally publishedYes


  • echogenic liposomes
  • microbubbles
  • ultrasound contrast agents


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