Macromolecular Interactions in Cartilage Extracellular Matrix Vary According to the Cartilage Type and Location

Manula S.B. Rathnayake, Brooke L. Farrugia, Karyna Kulakova, Colet E.M. ter Voert, Gerjo J.V.M. van Osch, Kathryn S. Stok*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

10 Downloads (Pure)


Objective: To investigate GAG-ECM (glycosaminoglycan–extracellular matrix) interactions in different cartilage types. To achieve this, we first aimed to determine protocols for consistent calculation of GAG content between cartilage types. Design: Auricular cartilage containing both collagen and elastin was used to determine the effect of lyophilization on GAG depletion activity. Bovine articular, auricular, meniscal, and nasal cartilage plugs were treated using different reagents to selectively remove GAGs. Sulfated glycosaminoglycan (sGAG) remaining in the sample after treatment were measured, and sGAG loss was compared between cartilage types. Results: The results indicate that dry weight of cartilage should be measured prior to cartilage treatment in order to provide a more accurate reference for normalization. Articular, meniscal, and nasal cartilage lost significant amounts of sGAG for all reagents used. However, only hyaluronidase was able to remove significant amount of sGAG from auricular cartilage. Furthermore, hyaluronidase was able to remove over 99% of sGAG from all cartilage types except auricular cartilage where it only removed around 76% of sGAG. The results indicate GAG-specific ECM binding for different cartilage types and locations. Conclusions: In conclusion, lyophilization can be performed to determine native dry weight for normalization without affecting the degree of GAG treatment. To our knowledge, this is the first study to compare GAG-ECM interactions of different cartilage types using different GAG extraction methods. Degree of GAG depletion not only varied with cartilage type but also the same type from different anatomic locations. This suggests specific structure-function roles for GAG populations found in the tissues.

Original languageEnglish
Pages (from-to)476S-485S
Number of pages10
Issue number2
Publication statusPublished - 2021

Bibliographical note

Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project

Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.


  • auricular
  • glycosaminoglycans
  • meniscus
  • nasal septal
  • proteoglycans


Dive into the research topics of 'Macromolecular Interactions in Cartilage Extracellular Matrix Vary According to the Cartilage Type and Location'. Together they form a unique fingerprint.

Cite this