MALDI TIMS IMS of Disialoganglioside Isomers GD1a and GD1b in Murine Brain Tissue

Katerina V. Djambazova; Martin Dufresne; Lukasz G. Migas; Angela R.S. Kruse; Raf Van De Plas; Richard M. Caprioli; Jeffrey M. Spraggins

doi:10.1021/acs.analchem.2c03939

MALDI TIMS IMS of Disialoganglioside Isomers GD1a and GD1b in Murine Brain Tissue

Katerina V. Djambazova, Martin Dufresne, Lukasz G. Migas, Angela R.S. Kruse, Raf Van De Plas, Richard M. Caprioli, Jeffrey M. Spraggins^*

^*Corresponding author for this work

Team Raf Van de Plas

Research output: Contribution to journal › Article › Scientific › peer-review

7 Citations (Scopus)

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Abstract

Gangliosides are acidic glycosphingolipids, containing ceramide moieties and oligosaccharide chains with one or more sialic acid residue(s) and are highly diverse isomeric structures with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) enables the untargeted spatial analysis of gangliosides, among other biomolecules, directly from tissue sections. Integrating trapped ion mobility spectrometry with MALDI IMS allows for the analysis of isomeric lipid structures in situ. Here, we demonstrate the gas-phase separation and identification of disialoganglioside isomers GD1a and GD1b that differ in the position of a sialic acid residue, in multiple samples, including a standard mixture of both isomers, a biological extract, and directly from thin tissue sections. The unique spatial distributions of GD1a/b (d36:1) and GD1a/b (d38:1) isomers were determined in rat hippocampus and spinal cord tissue sections, demonstrating the ability to structurally characterize and spatially map gangliosides based on both the carbohydrate chain and ceramide moieties.

Original language	English
Pages (from-to)	1176-1183
Journal	Analytical Chemistry
Volume	95
Issue number	2
DOIs	https://doi.org/10.1021/acs.analchem.2c03939
Publication status	Published - 2023

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Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care
Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

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@article{df9415a136d54614880a615d054eae9b,

title = "MALDI TIMS IMS of Disialoganglioside Isomers GD1a and GD1b in Murine Brain Tissue",

abstract = "Gangliosides are acidic glycosphingolipids, containing ceramide moieties and oligosaccharide chains with one or more sialic acid residue(s) and are highly diverse isomeric structures with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) enables the untargeted spatial analysis of gangliosides, among other biomolecules, directly from tissue sections. Integrating trapped ion mobility spectrometry with MALDI IMS allows for the analysis of isomeric lipid structures in situ. Here, we demonstrate the gas-phase separation and identification of disialoganglioside isomers GD1a and GD1b that differ in the position of a sialic acid residue, in multiple samples, including a standard mixture of both isomers, a biological extract, and directly from thin tissue sections. The unique spatial distributions of GD1a/b (d36:1) and GD1a/b (d38:1) isomers were determined in rat hippocampus and spinal cord tissue sections, demonstrating the ability to structurally characterize and spatially map gangliosides based on both the carbohydrate chain and ceramide moieties.",

author = "Djambazova, {Katerina V.} and Martin Dufresne and Migas, {Lukasz G.} and Kruse, {Angela R.S.} and {Van De Plas}, Raf and Caprioli, {Richard M.} and Spraggins, {Jeffrey M.}",

note = "Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.",

year = "2023",

doi = "10.1021/acs.analchem.2c03939",

language = "English",

volume = "95",

pages = "1176--1183",

journal = "Analytical Chemistry",

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publisher = "American Chemical Society (ACS)",

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T1 - MALDI TIMS IMS of Disialoganglioside Isomers GD1a and GD1b in Murine Brain Tissue

AU - Djambazova, Katerina V.

AU - Dufresne, Martin

AU - Migas, Lukasz G.

AU - Kruse, Angela R.S.

AU - Van De Plas, Raf

AU - Caprioli, Richard M.

AU - Spraggins, Jeffrey M.

N1 - Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

PY - 2023

Y1 - 2023

N2 - Gangliosides are acidic glycosphingolipids, containing ceramide moieties and oligosaccharide chains with one or more sialic acid residue(s) and are highly diverse isomeric structures with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) enables the untargeted spatial analysis of gangliosides, among other biomolecules, directly from tissue sections. Integrating trapped ion mobility spectrometry with MALDI IMS allows for the analysis of isomeric lipid structures in situ. Here, we demonstrate the gas-phase separation and identification of disialoganglioside isomers GD1a and GD1b that differ in the position of a sialic acid residue, in multiple samples, including a standard mixture of both isomers, a biological extract, and directly from thin tissue sections. The unique spatial distributions of GD1a/b (d36:1) and GD1a/b (d38:1) isomers were determined in rat hippocampus and spinal cord tissue sections, demonstrating the ability to structurally characterize and spatially map gangliosides based on both the carbohydrate chain and ceramide moieties.

AB - Gangliosides are acidic glycosphingolipids, containing ceramide moieties and oligosaccharide chains with one or more sialic acid residue(s) and are highly diverse isomeric structures with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) enables the untargeted spatial analysis of gangliosides, among other biomolecules, directly from tissue sections. Integrating trapped ion mobility spectrometry with MALDI IMS allows for the analysis of isomeric lipid structures in situ. Here, we demonstrate the gas-phase separation and identification of disialoganglioside isomers GD1a and GD1b that differ in the position of a sialic acid residue, in multiple samples, including a standard mixture of both isomers, a biological extract, and directly from thin tissue sections. The unique spatial distributions of GD1a/b (d36:1) and GD1a/b (d38:1) isomers were determined in rat hippocampus and spinal cord tissue sections, demonstrating the ability to structurally characterize and spatially map gangliosides based on both the carbohydrate chain and ceramide moieties.

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MALDI TIMS IMS of Disialoganglioside Isomers GD1a and GD1b in Murine Brain Tissue

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