Membrane-bound electron transport systems of an anammox bacterium: A complexome analysis

Naomi M. de Almeida, Hans J C T Wessels, Rob M. de Graaf, Christina Ferousi, Mike S M Jetten, Jan T. Keltjens, Boran Kartal

Research output: Contribution to journalArticleScientificpeer-review

46 Citations (Scopus)

Abstract

Electron transport, or oxidative phosphorylation, is one of the hallmarks of life. To this end, prokaryotes evolved a vast variety of protein complexes, only a small part of which have been discovered and studied. These protein complexes allow them to occupy virtually every ecological niche on Earth. Here, we applied the method of proteomics-based complexome profiling to get a better understanding of the electron transport systems of the anaerobic ammonium-oxidizing (anammox) bacteria, the N2-producing key players of the global nitrogen cycle. By this method nearly all respiratory complexes that were previously predicted from genome analysis to be involved in energy and cell carbon fixation were validated. More importantly, new and unexpected ones were discovered. We believe that complexome profiling in concert with (meta)genomics offers great opportunities to expand our knowledge on bacterial respiratory processes at a rapid and massive pace, in particular in new and thus far poorly investigated non-model and environmentally-relevant species.

Original languageEnglish
Pages (from-to)1694-1704
Number of pages11
JournalBBA - Bioenergetics
Volume1857
Issue number10
DOIs
Publication statusPublished - 2016

Keywords

  • Anaerobic ammonium oxidation (anammox)
  • Blue Native gel electrophoresis
  • Complexome profiling
  • Respiratory complexes

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