TY - JOUR
T1 - Memory CD8+ T cell heterogeneity is primarily driven by pathogen-specific cues and additionally shaped by the tissue environment
AU - van der Gracht, Esmé T.I.
AU - Beyrend, Guillaume
AU - Abdelaal, Tamim
AU - Pardieck, Iris N.
AU - Wesselink, Thomas H.
AU - van Haften, Floortje J.
AU - van Duikeren, Suzanne
AU - Koning, Frits
AU - Arens, Ramon
PY - 2021
Y1 - 2021
N2 - SummaryFactors that govern the complex formation of memory T cells are not completelyunderstood. A better understanding of thedevelopment of memory Tcell hetero-geneity is however required to enhance vaccination and immunotherapy ap-proaches. Here we examined the impact of pathogen- and tissue-specific cueson memory CD8+T cell heterogeneity using high-dimensional single-cell mass cy-tometry and a tailored bioinformatics pipeline. We identified distinct populationsof pathogen-specific CD8+T cells that uniquely connected to a specific pathogenor associated to multiple types of acute and persistent infections. In addition, thetissue environment shaped the memory CD8+T cell heterogeneity, albeit to alesser extent than infection. The programming of memory CD8+T cell differenti-ation during acute infection is eventually superseded by persistent infection.Thus, the plethora of distinct memory CD8+T cell subsets that arise upon infec-tion is dominantly sculpted by the pathogen-specific cues and further shaped by the tissue environment.
AB - SummaryFactors that govern the complex formation of memory T cells are not completelyunderstood. A better understanding of thedevelopment of memory Tcell hetero-geneity is however required to enhance vaccination and immunotherapy ap-proaches. Here we examined the impact of pathogen- and tissue-specific cueson memory CD8+T cell heterogeneity using high-dimensional single-cell mass cy-tometry and a tailored bioinformatics pipeline. We identified distinct populationsof pathogen-specific CD8+T cells that uniquely connected to a specific pathogenor associated to multiple types of acute and persistent infections. In addition, thetissue environment shaped the memory CD8+T cell heterogeneity, albeit to alesser extent than infection. The programming of memory CD8+T cell differenti-ation during acute infection is eventually superseded by persistent infection.Thus, the plethora of distinct memory CD8+T cell subsets that arise upon infec-tion is dominantly sculpted by the pathogen-specific cues and further shaped by the tissue environment.
KW - Cell Biology
KW - Immunology
UR - http://www.scopus.com/inward/record.url?scp=85098723412&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2020.101954
DO - 10.1016/j.isci.2020.101954
M3 - Article
AN - SCOPUS:85098723412
SN - 2589-0042
VL - 24
JO - iScience
JF - iScience
IS - 1
M1 - 101954
ER -