Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis

Abdulkadir Yayci, Tim Dirks, Friederike Kogelheide, Miguel Alcalde, Frank Hollmann, Peter Awakowicz, Julia E. Bandow*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)
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The use of a microscale atmospheric pressure plasma jet (μAPPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the μAPPJ offered significantly higher H2O2 production rates and better handling of larger reaction volumes. The performance of the μAPPJ was evaluated with recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Using plasma-treated buffer, no side reactions with other plasma-generated species were detected. For long-term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the μAPPJ. The enzyme had a turnover of 36,415 mol mol−1 and retained almost full activity even after prolonged plasma treatment. Overall, the μAPPJ presents a promising plasma source for plasma-driven biocatalysis.

Original languageEnglish
Pages (from-to)5893-5897
Issue number23
Publication statusPublished - 2020


  • atmospheric pressure plasma
  • biocatalysis
  • hydrogen peroxide
  • peroxygenase
  • plasma chemistry


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