Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis

Abdulkadir Yayci; Tim Dirks; Friederike Kogelheide; Miguel Alcalde; Frank Hollmann; Peter Awakowicz; Julia E. Bandow

doi:10.1002/cctc.202001225

Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis

Abdulkadir Yayci, Tim Dirks, Friederike Kogelheide, Miguel Alcalde, Frank Hollmann, Peter Awakowicz, Julia E. Bandow^*

^*Corresponding author for this work

BT/Biocatalysis

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

The use of a microscale atmospheric pressure plasma jet (μAPPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the μAPPJ offered significantly higher H₂O₂ production rates and better handling of larger reaction volumes. The performance of the μAPPJ was evaluated with recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Using plasma-treated buffer, no side reactions with other plasma-generated species were detected. For long-term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the μAPPJ. The enzyme had a turnover of 36,415 mol mol⁻¹ and retained almost full activity even after prolonged plasma treatment. Overall, the μAPPJ presents a promising plasma source for plasma-driven biocatalysis.

Original language	English
Pages (from-to)	5893-5897
Journal	ChemCatChem
Volume	12
Issue number	23
DOIs	https://doi.org/10.1002/cctc.202001225
Publication status	Published - 2020

Keywords

atmospheric pressure plasma
biocatalysis
hydrogen peroxide
peroxygenase
plasma chemistry

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cctc.202001225Final published version, 1.87 MBLicence: CC BY

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title = "Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis",

abstract = "The use of a microscale atmospheric pressure plasma jet (μAPPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the μAPPJ offered significantly higher H2O2 production rates and better handling of larger reaction volumes. The performance of the μAPPJ was evaluated with recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Using plasma-treated buffer, no side reactions with other plasma-generated species were detected. For long-term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the μAPPJ. The enzyme had a turnover of 36,415 mol mol−1 and retained almost full activity even after prolonged plasma treatment. Overall, the μAPPJ presents a promising plasma source for plasma-driven biocatalysis.",

keywords = "atmospheric pressure plasma, biocatalysis, hydrogen peroxide, peroxygenase, plasma chemistry",

author = "Abdulkadir Yayci and Tim Dirks and Friederike Kogelheide and Miguel Alcalde and Frank Hollmann and Peter Awakowicz and Bandow, {Julia E.}",

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doi = "10.1002/cctc.202001225",

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T1 - Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis

AU - Yayci, Abdulkadir

AU - Dirks, Tim

AU - Kogelheide, Friederike

AU - Alcalde, Miguel

AU - Hollmann, Frank

AU - Awakowicz, Peter

AU - Bandow, Julia E.

PY - 2020

Y1 - 2020

N2 - The use of a microscale atmospheric pressure plasma jet (μAPPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the μAPPJ offered significantly higher H2O2 production rates and better handling of larger reaction volumes. The performance of the μAPPJ was evaluated with recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Using plasma-treated buffer, no side reactions with other plasma-generated species were detected. For long-term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the μAPPJ. The enzyme had a turnover of 36,415 mol mol−1 and retained almost full activity even after prolonged plasma treatment. Overall, the μAPPJ presents a promising plasma source for plasma-driven biocatalysis.

AB - The use of a microscale atmospheric pressure plasma jet (μAPPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the μAPPJ offered significantly higher H2O2 production rates and better handling of larger reaction volumes. The performance of the μAPPJ was evaluated with recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Using plasma-treated buffer, no side reactions with other plasma-generated species were detected. For long-term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the μAPPJ. The enzyme had a turnover of 36,415 mol mol−1 and retained almost full activity even after prolonged plasma treatment. Overall, the μAPPJ presents a promising plasma source for plasma-driven biocatalysis.

KW - atmospheric pressure plasma

KW - biocatalysis

KW - hydrogen peroxide

KW - peroxygenase

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Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis

Abstract

Keywords

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