Mimicking the nuclear pore complex using nanopores

Adithya Ananth

doi:10.4233/uuid:4f0141e7-9b97-4ed4-86be-9b885b420423

Mimicking the nuclear pore complex using nanopores

Adithya Ananth

BN/Cees Dekker Lab

Research output: Thesis › Dissertation (TU Delft)

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Abstract

Nuclear pore complexes acts as a gatekeeper for molecular transport between the nucleus and the cytoplasm in eukaryotic cells. The central NPC channel is filled with intrinsically disordered FG domains (phenylalanine (F), glycine (G)) that are responsible for the fascinating selectivity of NPCs, for which the underlying mechanism is still under considerable debate. In this thesis, a minimalistic mimic of (NPCs) was constructed using solid-state nanopore and DNA origami to study the spatial arrangement and transport process.

Original language	English
Awarding Institution	Delft University of Technology
Supervisors/Advisors	Dekker, C., Supervisor
Award date	10 Apr 2018
Print ISBNs	9789085933427
DOIs	https://doi.org/10.4233/uuid:4f0141e7-9b97-4ed4-86be-9b885b420423
Publication status	Published - 2018

Keywords

Single-molecule
solid-state nanopore
nuclear pore complex
proteins
ionic conductance
DNA origami
surface chemistry

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10.4233/uuid:4f0141e7-9b97-4ed4-86be-9b885b420423

PhD thesis_AnanthFinal published version, 11.2 MB

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AB - Nuclear pore complexes acts as a gatekeeper for molecular transport between the nucleus and the cytoplasm in eukaryotic cells. The central NPC channel is filled with intrinsically disordered FG domains (phenylalanine (F), glycine (G)) that are responsible for the fascinating selectivity of NPCs, for which the underlying mechanism is still under considerable debate. In this thesis, a minimalistic mimic of (NPCs) was constructed using solid-state nanopore and DNA origami to study the spatial arrangement and transport process.

KW - Single-molecule

KW - solid-state nanopore

KW - nuclear pore complex

KW - proteins

KW - ionic conductance

KW - DNA origami

KW - surface chemistry

UR - http://resolver.tudelft.nl/uuid:4f0141e7-9b97-4ed4-86be-9b885b420423

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ER -

Mimicking the nuclear pore complex using nanopores

Abstract

Keywords

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