Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level

A. Mareike Schmidt, Stephan R. Fagerer, Konstantins Jefimovs, Florian Buettner, Christian Marro, Erdem C. Siringil, Karl L. Boehlen, Martin Pabst, Alfredo J. Ibáñez

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different days and samples. By combining multiple measurements, we were able to identify three co-existing phenotypes in an isogenic population of Saccharomyces cerevisiae characterized by distinctively different levels of glycolytic intermediates.

Original languageEnglish
Pages (from-to)5709-5717
JournalAnalyst
Volume139
Issue number22
DOIs
Publication statusPublished - 2014
Externally publishedYes

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