mRNA structural dynamics shape Argonaute-target interactions

Suzan Ruijtenberg, Stijn Sonneveld, Tao Ju Cui, Ive Logister, Dion de Steenwinkel, Yao Xiao, Ian J. MacRae, Chirlmin Joo, Marvin E. Tanenbaum

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

Small interfering RNAs (siRNAs) promote RNA degradation in a variety of processes and have important clinical applications. siRNAs direct cleavage of target RNAs by guiding Argonaute2 (AGO2) to its target site. Target site accessibility is critical for AGO2-target interactions, but how target site accessibility is controlled in vivo is poorly understood. Here, we use live-cell single-molecule imaging in human cells to determine rate constants of the AGO2 cleavage cycle in vivo. We find that the rate-limiting step in mRNA cleavage frequently involves unmasking of target sites by translating ribosomes. Target site masking is caused by heterogeneous intramolecular RNA-RNA interactions, which can conceal target sites for many minutes in the absence of translation. Our results uncover how dynamic changes in mRNA structure shape AGO2-target recognition, provide estimates of mRNA folding and unfolding rates in vivo, and provide experimental evidence for the role of mRNA structural dynamics in control of mRNA-protein interactions.

Original languageEnglish
Pages (from-to)790-801
JournalNature Structural and Molecular Biology
Volume27
Issue number9
DOIs
Publication statusPublished - 2020

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