Multi-color electron microscopy by element-guided identification of cells, organelles and molecules

Marijke Scotuzzi; Jeroen Kuipers; Dasha I. Wensveen; Pascal De Boer; Kees C.W. Hagen; Jacob P. Hoogenboom; Ben N.G. Giepmans

doi:10.1038/srep45970

Multi-color electron microscopy by element-guided identification of cells, organelles and molecules

Marijke Scotuzzi, Jeroen Kuipers, Dasha I. Wensveen, Pascal De Boer, Kees C.W. Hagen, Jacob P. Hoogenboom, Ben N.G. Giepmans^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

35 Citations (Scopus)

61 Downloads (Pure)

Abstract

Cellular complexity is unraveled at nanometer resolution using electron microscopy (EM), but interpretation of macromolecular functionality is hampered by the difficulty in interpreting grey-scale images and the unidentified molecular content. We perform large-scale EM on mammalian tissue complemented with energy-dispersive X-ray analysis (EDX) to allow EM-data analysis based on elemental composition. Endogenous elements, labels (gold and cadmium-based nanoparticles) as well as stains are analyzed at ultrastructural resolution. This provides a wide palette of colors to paint the traditional grey-scale EM images for composition-based interpretation. Our proof-of-principle application of EM-EDX reveals that endocrine and exocrine vesicles exist in single cells in Islets of Langerhans. This highlights how elemental mapping reveals unbiased biomedical relevant information. Broad application of EM-EDX will further allow experimental analysis on large-scale tissue using endogenous elements, multiple stains, and multiple markers and thus brings nanometer-scale 'color-EM' as a promising tool to unravel molecular (de)regulation in biomedicine.

Original language	English
Article number	45970
Number of pages	8
Journal	Scientific Reports
Volume	7
DOIs	https://doi.org/10.1038/srep45970
Publication status	Published - 7 Apr 2017

Access to Document

10.1038/srep45970

srep45970Final published version, 3.9 MBLicence: CC BY-NC-ND

Cite this

@article{4da1eff4cfcf44848ff9a001082ec912,

title = "Multi-color electron microscopy by element-guided identification of cells, organelles and molecules",

abstract = "Cellular complexity is unraveled at nanometer resolution using electron microscopy (EM), but interpretation of macromolecular functionality is hampered by the difficulty in interpreting grey-scale images and the unidentified molecular content. We perform large-scale EM on mammalian tissue complemented with energy-dispersive X-ray analysis (EDX) to allow EM-data analysis based on elemental composition. Endogenous elements, labels (gold and cadmium-based nanoparticles) as well as stains are analyzed at ultrastructural resolution. This provides a wide palette of colors to paint the traditional grey-scale EM images for composition-based interpretation. Our proof-of-principle application of EM-EDX reveals that endocrine and exocrine vesicles exist in single cells in Islets of Langerhans. This highlights how elemental mapping reveals unbiased biomedical relevant information. Broad application of EM-EDX will further allow experimental analysis on large-scale tissue using endogenous elements, multiple stains, and multiple markers and thus brings nanometer-scale 'color-EM' as a promising tool to unravel molecular (de)regulation in biomedicine.",

author = "Marijke Scotuzzi and Jeroen Kuipers and Wensveen, {Dasha I.} and {De Boer}, Pascal and Hagen, {Kees C.W.} and Hoogenboom, {Jacob P.} and Giepmans, {Ben N.G.}",

year = "2017",

month = apr,

day = "7",

doi = "10.1038/srep45970",

language = "English",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature",

}

TY - JOUR

T1 - Multi-color electron microscopy by element-guided identification of cells, organelles and molecules

AU - Scotuzzi, Marijke

AU - Kuipers, Jeroen

AU - Wensveen, Dasha I.

AU - De Boer, Pascal

AU - Hagen, Kees C.W.

AU - Hoogenboom, Jacob P.

AU - Giepmans, Ben N.G.

PY - 2017/4/7

Y1 - 2017/4/7

N2 - Cellular complexity is unraveled at nanometer resolution using electron microscopy (EM), but interpretation of macromolecular functionality is hampered by the difficulty in interpreting grey-scale images and the unidentified molecular content. We perform large-scale EM on mammalian tissue complemented with energy-dispersive X-ray analysis (EDX) to allow EM-data analysis based on elemental composition. Endogenous elements, labels (gold and cadmium-based nanoparticles) as well as stains are analyzed at ultrastructural resolution. This provides a wide palette of colors to paint the traditional grey-scale EM images for composition-based interpretation. Our proof-of-principle application of EM-EDX reveals that endocrine and exocrine vesicles exist in single cells in Islets of Langerhans. This highlights how elemental mapping reveals unbiased biomedical relevant information. Broad application of EM-EDX will further allow experimental analysis on large-scale tissue using endogenous elements, multiple stains, and multiple markers and thus brings nanometer-scale 'color-EM' as a promising tool to unravel molecular (de)regulation in biomedicine.

AB - Cellular complexity is unraveled at nanometer resolution using electron microscopy (EM), but interpretation of macromolecular functionality is hampered by the difficulty in interpreting grey-scale images and the unidentified molecular content. We perform large-scale EM on mammalian tissue complemented with energy-dispersive X-ray analysis (EDX) to allow EM-data analysis based on elemental composition. Endogenous elements, labels (gold and cadmium-based nanoparticles) as well as stains are analyzed at ultrastructural resolution. This provides a wide palette of colors to paint the traditional grey-scale EM images for composition-based interpretation. Our proof-of-principle application of EM-EDX reveals that endocrine and exocrine vesicles exist in single cells in Islets of Langerhans. This highlights how elemental mapping reveals unbiased biomedical relevant information. Broad application of EM-EDX will further allow experimental analysis on large-scale tissue using endogenous elements, multiple stains, and multiple markers and thus brings nanometer-scale 'color-EM' as a promising tool to unravel molecular (de)regulation in biomedicine.

UR - http://resolver.tudelft.nl/uuid:4da1eff4-cfcf-4484-8ff9-a001082ec912

UR - http://www.scopus.com/inward/record.url?scp=85017218537&partnerID=8YFLogxK

U2 - 10.1038/srep45970

DO - 10.1038/srep45970

M3 - Article

AN - SCOPUS:85017218537

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 45970

ER -

Multi-color electron microscopy by element-guided identification of cells, organelles and molecules

Abstract

Access to Document

Other files and links

Fingerprint

Cite this