Multiplex genome editing of microorganisms using CRISPR-Cas

Belén Adiego-Pérez, Paola Randazzo, Jean Marc Daran, René Verwaal, Johannes A. Roubos, Pascale Daran-Lapujade, John Van Der Oost*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

63 Citations (Scopus)
177 Downloads (Pure)


Microbial production of chemical compounds often requires highly engineered microbial cell factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful tools for genome editing. Here, we briefly review the most frequently used CRISPR-Cas tools and describe some of their applications. We describe the progress made with respect to CRISPR-based multiplex genome editing of industrial bacteria and eukaryotic microorganisms. We also review the state of the art in terms of gene expression regulation using CRISPRi and CRISPRa. Finally, we summarize the pillars for efficient multiplexed genome editing and present our view on future developments and applications of CRISPR-Cas tools for multiplex genome editing.

Original languageEnglish
Article numberfnz086
Number of pages19
JournalFEMS Microbiology Letters
Issue number8
Publication statusPublished - 2019


  • Cas12a
  • Cas9
  • Cell factories
  • CRISPR-Cas
  • Genome editing
  • Multiplex


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