TY - JOUR
T1 - Multiresidue determination of antibiotics in preserved eggs using a QuEChERS-based procedure by ultrahigh-performance liquid chromatography tandem mass spectrometry
AU - Li, Yonggang
AU - Chen, Ziliang
AU - Wen, Sheng
AU - Hou, Xiaolin
AU - Zhang, Rui
AU - Ma, Meihu
PY - 2018
Y1 - 2018
N2 - A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method and ultrahigh-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) were optimized and validated for 16 antibiotics belonging to three families (macrolides, quinolones, and sulfonamides) that were found in preserved eggs. Samples were extracted in 4 mL water and 10 mL acetonitrile with 1% acetic acid and subjected to a cleanup procedure using dispersive solid-phase extraction with C18 and primary secondary amine sorbents, prior to detection by UHPLC-MS/MS. Matrix-matched calibration was used for quantification to reduce the matrix effect with limits of quantification in the range of 0.3-3.0 μg/kg. Validation of the method was conducted by recovery and precision experiments. Recoveries of the spiked samples ranged from 73.8% to 127.4%, and the intra- and inter-day relative standard deviations were lower than 21.2% and 22.3%, respectively. This method was successfully applied to the analysis of antibiotics in preserved egg samples.
AB - A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method and ultrahigh-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) were optimized and validated for 16 antibiotics belonging to three families (macrolides, quinolones, and sulfonamides) that were found in preserved eggs. Samples were extracted in 4 mL water and 10 mL acetonitrile with 1% acetic acid and subjected to a cleanup procedure using dispersive solid-phase extraction with C18 and primary secondary amine sorbents, prior to detection by UHPLC-MS/MS. Matrix-matched calibration was used for quantification to reduce the matrix effect with limits of quantification in the range of 0.3-3.0 μg/kg. Validation of the method was conducted by recovery and precision experiments. Recoveries of the spiked samples ranged from 73.8% to 127.4%, and the intra- and inter-day relative standard deviations were lower than 21.2% and 22.3%, respectively. This method was successfully applied to the analysis of antibiotics in preserved egg samples.
KW - Antibiotics
KW - Macrolides
KW - Preserved eggs
KW - QuEChERS
KW - Quinolones
KW - Sulfonamides
KW - UHPLC-MS/MS
UR - http://resolver.tudelft.nl/uuid:35ed54eb-6be0-4d11-9201-0786f4f9d9cc
UR - http://www.scopus.com/inward/record.url?scp=85045873242&partnerID=8YFLogxK
U2 - 10.1556/1326.2017.29211
DO - 10.1556/1326.2017.29211
M3 - Article
AN - SCOPUS:85045873242
VL - 30
SP - 9
EP - 16
JO - Acta Chromatographica
JF - Acta Chromatographica
SN - 1233-2356
IS - 1
ER -