TY - JOUR
T1 - Natural deep eutectic solvents as biofilm structural breakers
AU - Nava-Ocampo, Maria F.
AU - Fuhaid, Lamya Al
AU - Verpoorte, Robert
AU - Choi, Young Hae
AU - van Loosdrecht, Mark C.M.
AU - Vrouwenvelder, Johannes S.
AU - Witkamp, Geert J.
AU - Farinha, Andreia S.F.
AU - Bucs, Szilárd S.
PY - 2021
Y1 - 2021
N2 - Natural Deep Eutectic Solvents (NADES) are composed of supramolecular interactions of two or more natural compounds, such as organic acids, sugars, and amino acids, and they are being used as a new media alternative to conventional solvents. In this study, a new application of NADES is presented as a possible technology for biofilm structural breaker in complex systems since the current solvents used for biofilm cleaning and extraction of biofilm components use hazardous solutions. The NADES (betaine:urea:water and lactic acid:glucose:water) were analyzed before and after the biofilm treatment by attenuated total reflection Fourier-transform infrared spectroscopy and fluorescence excitation-emission matrix spectroscopy. Our results indicate that the green solvents could solubilize up to ≈70 percent of the main components of the biofilms extracellular matrix. The solubilization of the biomolecules weakened the biofilm structure, which could enhance the biofilm solubilization and removal. The NADES have the potential to be an environment-friendly, green solvent to extract valuable compounds and break the main structure from the biofilm, leading to a greener method for extracellular polymeric substance (EPS) extraction and biofilm treatment in various water treatment systems.
AB - Natural Deep Eutectic Solvents (NADES) are composed of supramolecular interactions of two or more natural compounds, such as organic acids, sugars, and amino acids, and they are being used as a new media alternative to conventional solvents. In this study, a new application of NADES is presented as a possible technology for biofilm structural breaker in complex systems since the current solvents used for biofilm cleaning and extraction of biofilm components use hazardous solutions. The NADES (betaine:urea:water and lactic acid:glucose:water) were analyzed before and after the biofilm treatment by attenuated total reflection Fourier-transform infrared spectroscopy and fluorescence excitation-emission matrix spectroscopy. Our results indicate that the green solvents could solubilize up to ≈70 percent of the main components of the biofilms extracellular matrix. The solubilization of the biomolecules weakened the biofilm structure, which could enhance the biofilm solubilization and removal. The NADES have the potential to be an environment-friendly, green solvent to extract valuable compounds and break the main structure from the biofilm, leading to a greener method for extracellular polymeric substance (EPS) extraction and biofilm treatment in various water treatment systems.
KW - aerobic granular sludge
KW - biofilm structural modifiers
KW - green solvents
KW - Natural Deep Eutectic Solvents
UR - http://www.scopus.com/inward/record.url?scp=85109011359&partnerID=8YFLogxK
U2 - 10.1016/j.watres.2021.117323
DO - 10.1016/j.watres.2021.117323
M3 - Article
C2 - 34139511
AN - SCOPUS:85109011359
SN - 0043-1354
VL - 201
JO - Water Research
JF - Water Research
M1 - 117323
ER -