TY - JOUR
T1 - Navigating the crowd
T2 - visualizing coordination between genome dynamics, structure, and transcription
AU - Shaban, Haitham A.
AU - Barth, Roman
AU - Bystricky, Kerstin
PY - 2020
Y1 - 2020
N2 - The eukaryotic genome is hierarchically structured yet highly dynamic. Regulating transcription in this environment demands a high level of coordination to permit many proteins to interact with chromatin fiber at appropriate sites in a timely manner. We describe how recent advances in quantitative imaging techniques overcome caveats of sequencing-based methods (Hi-C and related) by enabling direct visualization of transcription factors and chromatin at high resolution, from single genes to the whole nucleus. We discuss the contribution of fluorescence imaging to deciphering the principles underlying this coordination within the crowded nuclear space in living cells and discuss challenges ahead.
AB - The eukaryotic genome is hierarchically structured yet highly dynamic. Regulating transcription in this environment demands a high level of coordination to permit many proteins to interact with chromatin fiber at appropriate sites in a timely manner. We describe how recent advances in quantitative imaging techniques overcome caveats of sequencing-based methods (Hi-C and related) by enabling direct visualization of transcription factors and chromatin at high resolution, from single genes to the whole nucleus. We discuss the contribution of fluorescence imaging to deciphering the principles underlying this coordination within the crowded nuclear space in living cells and discuss challenges ahead.
UR - http://www.scopus.com/inward/record.url?scp=85096339987&partnerID=8YFLogxK
U2 - 10.1186/s13059-020-02185-y
DO - 10.1186/s13059-020-02185-y
M3 - Review article
C2 - 33203432
AN - SCOPUS:85096339987
VL - 21
JO - Genome Biology (Online)
JF - Genome Biology (Online)
SN - 1474-760X
IS - 1
M1 - 278
ER -