Navigating the crowd: visualizing coordination between genome dynamics, structure, and transcription

Haitham A. Shaban*, Roman Barth, Kerstin Bystricky

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

3 Citations (Scopus)
6 Downloads (Pure)

Abstract

The eukaryotic genome is hierarchically structured yet highly dynamic. Regulating transcription in this environment demands a high level of coordination to permit many proteins to interact with chromatin fiber at appropriate sites in a timely manner. We describe how recent advances in quantitative imaging techniques overcome caveats of sequencing-based methods (Hi-C and related) by enabling direct visualization of transcription factors and chromatin at high resolution, from single genes to the whole nucleus. We discuss the contribution of fluorescence imaging to deciphering the principles underlying this coordination within the crowded nuclear space in living cells and discuss challenges ahead.

Original languageEnglish
Article number278
Number of pages18
JournalGenome biology
Volume21
Issue number1
DOIs
Publication statusPublished - 2020

Fingerprint

Dive into the research topics of 'Navigating the crowd: visualizing coordination between genome dynamics, structure, and transcription'. Together they form a unique fingerprint.

Cite this