Non-marking Collection of Amino Acids from Fingerprints Using Hydrogels

Ward van Helmond, Vincent O’Brien, Robin de Jong, Jan van Esch, Sander Oldenhof, Marcel de Puit*

*Corresponding author for this work

Research output: Chapter in Book/Conference proceedings/Edited volumeChapterScientificpeer-review


The amino acid profile obtained from a fingerprint may provide valuable information on its donor. Unfortunately, the collection of chemicals from the fingerprint is often destructive to the fingerprint ridge detail. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualization at a later stage using the standard method of cyanoacrylate fuming followed by basic-yellow dyeing.

Original languageEnglish
Title of host publicationAMINO ACID ANALYSIS, 2 EDITION
EditorsJohn M. Walker
ISBN (Electronic)978-1-4939-9639-1-32
ISBN (Print)978-1-4939-9638-4
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Amino acid analysis
  • Fingerprint
  • Hydrogel
  • Non-marking


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