Optimal model-based sensorless adaptive optics for epifluorescence microscopy

Paolo Pozzi*, Oleg Soloviev, Dean Wilding, Gleb Vdovin, Michel Verhaegen

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)
49 Downloads (Pure)

Abstract

We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image quality and the aberration. The method is potentially applicable to any form of microscopy with epifluorescence detection, including the practically important case of incoherent fluorescence emission from a three dimensional object, through minor hardware modifications. We have applied the technique successfully to a widefield epifluorescence microscope and to a multiaperture confocal microscope.

Original languageEnglish
Article numbere0194523
Number of pages15
JournalPLoS ONE
Volume13
Issue number3
DOIs
Publication statusPublished - 2018

Keywords

  • OA-Fund TU Delft

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